EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of Venezuelan equine encephalitis virus with its receptor LDLRAD3.
ジャーナル・号・ページ	Nature, Vol. 598, Issue 7882, Page 677-681, Year 2021
掲載日	2021年10月13日
著者	Bingting Ma / Cuiqing Huang / Jun Ma / Ye Xiang / Xinzheng Zhang /
PubMed 要旨	Venezuelan equine encephalitis virus (VEEV) is an enveloped RNA virus that causes encephalitis and potentially mortality in infected humans and equines. At present, no vaccines or drugs are available ...Venezuelan equine encephalitis virus (VEEV) is an enveloped RNA virus that causes encephalitis and potentially mortality in infected humans and equines. At present, no vaccines or drugs are available that prevent or cure diseases caused by VEEV. Low-density lipoprotein receptor class A domain-containing 3 (LDLRAD3) was recently identified as a receptor for the entry of VEEV into host cells. Here we present the cryo-electron microscopy structure of the LDLRAD3 extracellular domain 1 (LDLRAD3-D1) in complex with VEEV virus-like particles at a resolution of 3.0 Å. LDLRAD3-D1 has a cork-like structure and is inserted into clefts formed between adjacent VEEV E2-E1 heterodimers in the viral-surface trimer spikes through hydrophobic and polar contacts. Mutagenesis studies of LDLRAD3-D1 identified residues that are involved in the key interactions with VEEV. Of note, some of the LDLRAD3-D1 mutants showed a significantly increased binding affinity for VEEV, suggesting that LDLRAD3-D1 may serve as a potential scaffold for the development of inhibitors of VEEV entry. Our structures provide insights into alphavirus assembly and the binding of receptors to alphaviruses, which may guide the development of therapeutic countermeasures against alphaviruses.
リンク	Nature / PubMed:34646021
手法	EM (単粒子)
解像度	3.0 - 3.5 Å
構造データ	31566 7ffe EMDB-31566, PDB-7ffe: Cryo-EM structure of VEEV VLP 手法: EM (単粒子) / 解像度: 3.5 Å 31567 7fff EMDB-31567, PDB-7fff: Structure of Venezuelan equine encephalitis virus with the receptor LDLRAD3 手法: EM (単粒子) / 解像度: 3.0 Å 31568 7ffl EMDB-31568, PDB-7ffl: Cryo-EM structure of VEEV VLP-LDLRAD3-D1 complex at the 2-fold axes 手法: EM (単粒子) / 解像度: 3.1 Å 31569 7ffn EMDB-31569, PDB-7ffn: Cryo-EM structure of VEEV VLP-LDLRAD3-D1 complex at the 5-fold axes 手法: EM (単粒子) / 解像度: 3.0 Å 31570 7ffo EMDB-31570, PDB-7ffo: Cryo-EM structure of VEEV VLP at the 5-fold axes 手法: EM (単粒子) / 解像度: 3.5 Å 31571 7ffq EMDB-31571, PDB-7ffq: Cryo-EM structure of VEEV VLP at the 2-fold axes 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	venezuelan equine encephalitis virus (strain tc-83) (ウイルス) homo sapiens (ヒト)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / Virus (ウイルス) / Receptor / Complex / VIRUS LIKE PARTICLE/PROTEIN BINDING / VIRUS LIKE PARTICLE-PROTEIN BINDING complex