+検索条件
-Structure paper
タイトル | Pseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 4204, Year 2022 |
掲載日 | 2022年7月20日 |
著者 | Dingwei He / Linlin You / Xiaoxian Wu / Jing Shi / Aijia Wen / Zhi Yan / Wenhui Mu / Chengli Fang / Yu Feng / Yu Zhang / |
PubMed 要旨 | Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme ...Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme comprising the stress-responsive σ factor σ (RNAP-σ). SutA shows no homology to previously characterized RNAP-binding proteins. The structure and mode of action of SutA remain unclear. Here we determined cryo-EM structures of Pae RNAP-σ holoenzyme, Pae RNAP-σ holoenzyme complexed with SutA, and Pae RNAP-σ transcription initiation complex comprising SutA. The structures show SutA pinches RNAP-β protrusion and facilitates promoter unwinding by wedging RNAP-β lobe open. Our results demonstrate that SutA clears an energetic barrier to facilitate promoter unwinding of RNAP-σ holoenzyme. |
リンク | Nat Commun / PubMed:35859063 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.13 - 5.8 Å |
構造データ | EMDB-31403, PDB-7f0r: EMDB-31948, PDB-7vf9: EMDB-33271, PDB-7xl3: EMDB-33272, PDB-7xl4: |
化合物 | ChemComp-ZN: ChemComp-MG: |
由来 |
|
キーワード | TRANSCRIPTION/DNA / transcription initiation / Pseudomonas aeruginosa / SutA / transcription activation / sigmaS / RNA polymerase / RNAP beta lobe / TRANSLATION / TRANSCRIPTION-DNA complex / TRANSCRIPTION / closed beta lobe / holoenzyme / open beta lobe |