タイトル | Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Page 2828-2828, Year 2021 |
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掲載日 | 2020年8月14日 (構造データの登録日) |
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著者 | Xiao, Y. / Shao, K. / Zhou, J. / Wang, L. / Ma, X. / Wu, D. / Yang, Y. / Chen, J. / Feng, J. / Qiu, S. ...Xiao, Y. / Shao, K. / Zhou, J. / Wang, L. / Ma, X. / Wu, D. / Yang, Y. / Chen, J. / Feng, J. / Qiu, S. / Lv, Z. / Zhang, L. / Zhang, P. / Chen, W. |
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リンク | Nat Commun / PubMed:33990581 |
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手法 | X線回折 |
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解像度 | 1.59077503881 - 2.80114138241 Å |
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構造データ | PDB-7cs2: Apo structure of dimeric IiPLR1 手法: X-RAY DIFFRACTION / 解像度: 2.68800468428 Å PDB-7cs3: IiPLR1 with NADP+ 手法: X-RAY DIFFRACTION / 解像度: 2.4002184429 Å PDB-7cs4: IiPLR1 with NADP+ and (+)pinoresinol 手法: X-RAY DIFFRACTION / 解像度: 2.3050924643 Å PDB-7cs5: IiPLR1 with NADP+ and (-)pinoresinol 手法: X-RAY DIFFRACTION / 解像度: 2.18993226397 Å PDB-7cs6: IiPLR1 with NADP+ and (-)lariciresinol 手法: X-RAY DIFFRACTION / 解像度: 2.20139658355 Å PDB-7cs7: IiPLR1 with NADP+ and (+)secoisolariciresinol 手法: X-RAY DIFFRACTION / 解像度: 2.29765287047 Å PDB-7cs8: IiPLR1 with NADP+ and (-)secoisolariciresinol 手法: X-RAY DIFFRACTION / 解像度: 2.60003253525 Å PDB-7cs9: AtPrR1 in apo form 手法: X-RAY DIFFRACTION / 解像度: 2.80114138241 Å PDB-7csa: AtPrR1 with NADP+ 手法: X-RAY DIFFRACTION / 解像度: 1.96212826124 Å PDB-7csb: AtPrR1 with NADP+ and (+)pinoresinol 手法: X-RAY DIFFRACTION / 解像度: 2.00201707558 Å PDB-7csc: AtPrR1 with NADP+ and (-)pinoresinol 手法: X-RAY DIFFRACTION / 解像度: 2.51511848629 Å PDB-7csd: AtPrR1 with NADP+ and (+)lariciresinol 手法: X-RAY DIFFRACTION / 解像度: 1.79709972859 Å PDB-7cse: AtPrR1 with NADP+ and (-)lariciresinol 手法: X-RAY DIFFRACTION / 解像度: 2.4401905733 Å PDB-7csf: AtPrR1 with NADP+ and (-)secoisolariciresinol 手法: X-RAY DIFFRACTION / 解像度: 1.98241803828 Å PDB-7csg: AtPrR2 in apo form 手法: X-RAY DIFFRACTION / 解像度: 1.99689386689 Å PDB-7csh: AtPrR2 with NADP+ and (+)pinoresinol 手法: X-RAY DIFFRACTION / 解像度: 1.59077503881 Å |
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化合物 | ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH
ChemComp-GEC: 4-[(3S,3aR,6S,6aR)-6-(3-methoxy-4-oxidanyl-phenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-3-yl]-2-methoxy-phenol / ピノレシノ-ル
ChemComp-GEF: 4-[(3R,3aS,6R,6aS)-6-(3-methoxy-4-oxidanyl-phenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-3-yl]-2-methoxy-phenol / (-)-ピノレシノ-ル
ChemComp-GFR: 4-[[(3S,4S,5R)-4-(hydroxymethyl)-5-(3-methoxy-4-oxidanyl-phenyl)oxolan-3-yl]methyl]-2-methoxy-phenol / (-)-ラリシレシノ-ル
ChemComp-GFU: 4-[[(3R,4R,5S)-4-(hydroxymethyl)-5-(3-methoxy-4-oxidanyl-phenyl)oxolan-3-yl]methyl]-2-methoxy-phenol / (+)-ラリシレシノ-ル
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由来 | - isatis tinctoria (ホソバタイセイ)
- arabidopsis thaliana (シロイヌナズナ)
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キーワード | OXIDOREDUCTASE / PLR1 / dimer / PLANT PROTEIN / IiPLR1 / NADP+ / pinoresinol / (-)pinoresinol / lariciresinol / secoisolariciresinol / AtPrR1 / (+)lariciresinol / (-)secoisolariciresinol / AtPrR2 / homodimer / (+)pinoresinol |
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