EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol.
ジャーナル・号・ページ	Science, Vol. 368, Issue 6496, Page 1211-1219, Year 2020
掲載日	2020年6月12日
著者	Lu Zhang / Yao Zhao / Yan Gao / Lijie Wu / Ruogu Gao / Qi Zhang / Yinan Wang / Chengyao Wu / Fangyu Wu / Sudagar S Gurcha / Natacha Veerapen / Sarah M Batt / Wei Zhao / Ling Qin / Xiuna Yang / Manfu Wang / Yan Zhu / Bing Zhang / Lijun Bi / Xian'en Zhang / Haitao Yang / Luke W Guddat / Wenqing Xu / Quan Wang / Jun Li / Gurdyal S Besra / Zihe Rao /
PubMed 要旨	The arabinosyltransferases EmbA, EmbB, and EmbC are involved in cell wall synthesis and are recognized as targets for the anti-tuberculosis drug ethambutol. In this study, we determined cryo- ...The arabinosyltransferases EmbA, EmbB, and EmbC are involved in cell wall synthesis and are recognized as targets for the anti-tuberculosis drug ethambutol. In this study, we determined cryo-electron microscopy and x-ray crystal structures of mycobacterial EmbA-EmbB and EmbC-EmbC complexes in the presence of their glycosyl donor and acceptor substrates and with ethambutol. These structures show how the donor and acceptor substrates bind in the active site and how ethambutol inhibits arabinosyltransferases by binding to the same site as both substrates in EmbB and EmbC. Most drug-resistant mutations are located near the ethambutol binding site. Collectively, our work provides a structural basis for understanding the biochemical function and inhibition of arabinosyltransferases and the development of new anti-tuberculosis agents.
リンク	Science / PubMed:32327601
手法	EM (単粒子) / X線回折
解像度	2.81 - 3.3 Å
構造データ	30216 7bvc EMDB-30216, PDB-7bvc: Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol 手法: EM (単粒子) / 解像度: 2.9 Å 30217 7bve EMDB-30217, PDB-7bve: Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbC2-AcpM2 in complex with ethambutol 手法: EM (単粒子) / 解像度: 2.81 Å 30218 7bvf EMDB-30218, PDB-7bvf: Cryo-EM structure of Mycobacterium tuberculosis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with ethambutol 手法: EM (単粒子) / 解像度: 2.97 Å 30219 7bvg EMDB-30219, PDB-7bvg: Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with di-arabinose. 手法: EM (単粒子) / 解像度: 3.1 Å PDB-7bvh: Crystal structure of arabinosyltransferase EmbC2-AcpM2 complex from Mycobacterium smegmatis complexed with di-arabinose 手法: X-RAY DIFFRACTION / 解像度: 3.3 Å
化合物	ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-F8L: [(2Z,6E,10E,14Z,18E,22Z,26Z)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl] [(2S,3S,4S,5R)-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl] hydrogen phosphate ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸 / Phosphopantetheine ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-95E: Ethambutol / エタンブト-ル / 薬剤YM / エタンブトール ChemComp-PN7: N~3~-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-sulfanylethyl)-beta-alaninamide ChemComp-DSL: MONO-TRANS, OCTA-CIS DECAPRENYL-PHOSPHATE ChemComp-BXY: alpha-D-arabinofuranose / α-D-アラビノフラノ-ス / アラビノース
由来	mycolicibacterium smegmatis mc2 155 (バクテリア) mycolicibacterium smegmatis (strain atcc 700084 / mc(2)155) (バクテリア) mycobacterium tuberculosis h37rv (結核菌)
キーワード	TRANSFERASE (転移酵素) / Mycobacterium smegmatis / cell wall synthesis / drug target (生物学的標的) / ethambutol (エタンブトール) / arabinosyltransferase / EmbA / EmbB / EmbC / acyl carrier protein / arabinogalactan / lipoarabinomannan / drug resistance (薬剤耐性) / Mycobacterium tuberculosis (結核菌)