EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 19, Year 2021
掲載日	2021年5月11日
著者	Dominik Hrebík / Tibor Füzik / Mária Gondová / Lenka Šmerdová / Athanassios Adamopoulos / Ondrej Šedo / Zbyněk Zdráhal / Pavel Plevka /
PubMed 要旨	Most rhinoviruses, which are the leading cause of the common cold, utilize intercellular adhesion molecule-1 (ICAM-1) as a receptor to infect cells. To release their genomes, rhinoviruses convert to ...Most rhinoviruses, which are the leading cause of the common cold, utilize intercellular adhesion molecule-1 (ICAM-1) as a receptor to infect cells. To release their genomes, rhinoviruses convert to activated particles that contain pores in the capsid, lack minor capsid protein VP4, and have an altered genome organization. The binding of rhinoviruses to ICAM-1 promotes virus activation; however, the molecular details of the process remain unknown. Here, we present the structures of virion of rhinovirus 14 and its complex with ICAM-1 determined to resolutions of 2.6 and 2.4 Å, respectively. The cryo-electron microscopy reconstruction of rhinovirus 14 virions contains the resolved density of octanucleotide segments from the RNA genome that interact with VP2 subunits. We show that the binding of ICAM-1 to rhinovirus 14 is required to prime the virus for activation and genome release at acidic pH. Formation of the rhinovirus 14-ICAM-1 complex induces conformational changes to the rhinovirus 14 capsid, including translocation of the C termini of VP4 subunits, which become poised for release through pores that open in the capsids of activated particles. VP4 subunits with altered conformation block the RNA-VP2 interactions and expose patches of positively charged residues. The conformational changes to the capsid induce the redistribution of the virus genome by altering the capsid-RNA interactions. The restructuring of the rhinovirus 14 capsid and genome prepares the virions for conversion to activated particles. The high-resolution structure of rhinovirus 14 in complex with ICAM-1 explains how the binding of uncoating receptors enables enterovirus genome release.
リンク	Proc Natl Acad Sci U S A / PubMed:33947819 / PubMed Central
手法	EM (単粒子)
解像度	2.4 - 22.0 Å
構造データ	12171 7bg6 EMDB-12171: HRV14 native particle PDB-7bg6: HRV14 native particle solved by cryoEM 手法: EM (単粒子) / 解像度: 2.6 Å 12172 7bg7 EMDB-12172, PDB-7bg7: HRV14 in complex with its receptor ICAM-1 手法: EM (単粒子) / 解像度: 2.4 Å 12594 7nul EMDB-12594, PDB-7nul: Rhinovirus-14 ICAM-1 activated particle at pH 6.2 手法: EM (単粒子) / 解像度: 4.0 Å 12595 7num EMDB-12595, PDB-7num: Rhinovirus-14 ICAM-1 empty particle at pH 6.2 手法: EM (単粒子) / 解像度: 3.9 Å 12596 7nun EMDB-12596, PDB-7nun: Rhinovirus 14 ICAM-1 virion-like particle at pH 6.2 手法: EM (単粒子) / 解像度: 3.6 Å 12597 7nuo EMDB-12597, PDB-7nuo: Rhinovirus 14 empty particle at pH 6.2 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-12598: RV14 ICAM-1 pH 6.2 open particle 手法: EM (単粒子) / 解像度: 22.0 Å 12599 7nuq EMDB-12599, PDB-7nuq: Rhinovirus 14 virion-like at pH 6.2 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-HOH: WATER / 水
由来	rhinovirus b14 (ライノウイルス) HRV-14 (ライノウイルス) human rhinovirus 14 (ライノウイルス) homo sapiens (ヒト)
キーワード	VIRUS (ウイルス) / enterovirus (エンテロウイルス) / rhinovirus 14 (ライノウイルス) / HRV14 / RV14 / native particle / receptor (受容体) / virus-receptor complex / ICAM-1 (ICAM-1) / acidification / pH 6.2 / genome release / A particle / activated / emptu / empty particle