EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The coupling mechanism of mammalian respiratory complex I.
ジャーナル・号・ページ	Science, Vol. 370, Issue 6516, Year 2020
掲載日	2020年10月30日
著者	Domen Kampjut / Leonid A Sazanov /
PubMed 要旨	Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different ...Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different conditions, including turnover, at resolutions up to 2.3 to 2.5 angstroms. Resolved water molecules allowed us to experimentally define the proton translocation pathways. Quinone binds at three positions along the quinone cavity, as does the inhibitor rotenone that also binds within subunit ND4. Dramatic conformational changes around the quinone cavity couple the redox reaction to proton translocation during open-to-closed state transitions of the enzyme. In the induced deactive state, the open conformation is arrested by the ND6 subunit. We propose a detailed molecular coupling mechanism of complex I, which is an unexpected combination of conformational changes and electrostatic interactions.
リンク	Science / PubMed:32972993
手法	EM (単粒子)
解像度	2.3 - 3.8 Å
構造データ	11241 6zk9 EMDB-11241, PDB-6zk9: Peripheral domain of open complex I during turnover 手法: EM (単粒子) / 解像度: 2.3 Å 11242 6zka EMDB-11242, PDB-6zka: Membrane domain of open complex I during turnover 手法: EM (単粒子) / 解像度: 2.5 Å 11243 6zkb EMDB-11243, PDB-6zkb: Membrane domain of closed complex I during turnover 手法: EM (単粒子) / 解像度: 2.9 Å 11244 6zkc EMDB-11244, PDB-6zkc: Complex I during turnover, closed 手法: EM (単粒子) / 解像度: 3.1 Å 11245 6zkd EMDB-11245, PDB-6zkd: Complex I during turnover, open1 手法: EM (単粒子) / 解像度: 2.7 Å 11246 6zke EMDB-11246, PDB-6zke: Complex I during turnover, open2 手法: EM (単粒子) / 解像度: 2.6 Å 11247 6zkf EMDB-11247, PDB-6zkf: Complex I during turnover, open3 手法: EM (単粒子) / 解像度: 2.8 Å 11248 6zkg EMDB-11248, PDB-6zkg: Complex I with NADH, closed 手法: EM (単粒子) / 解像度: 3.4 Å 11249 6zkh EMDB-11249, PDB-6zkh: Complex I with NADH, open1 手法: EM (単粒子) / 解像度: 3.0 Å 11250 6zki EMDB-11250, PDB-6zki: Complex I with NADH, open2 手法: EM (単粒子) / 解像度: 2.8 Å 11251 6zkj EMDB-11251, PDB-6zkj: Complex I with NADH, open3 手法: EM (単粒子) / 解像度: 3.0 Å 11252 6zkk EMDB-11252, PDB-6zkk: Complex I inhibited by rotenone, closed 手法: EM (単粒子) / 解像度: 3.7 Å 11253 6zkl EMDB-11253, PDB-6zkl: Complex I inhibited by rotenone, open1 手法: EM (単粒子) / 解像度: 3.1 Å 11254 6zkm EMDB-11254, PDB-6zkm: Complex I inhibited by rotenone, open2 手法: EM (単粒子) / 解像度: 2.8 Å 11255 6zkn EMDB-11255, PDB-6zkn: Complex I inhibited by rotenone, open3 手法: EM (単粒子) / 解像度: 2.9 Å 11256 6zko EMDB-11256, PDB-6zko: Native complex I, closed 手法: EM (単粒子) / 解像度: 3.8 Å 11257 6zkp EMDB-11257, PDB-6zkp: Native complex I, open1 手法: EM (単粒子) / 解像度: 3.2 Å 11258 6zkq EMDB-11258, PDB-6zkq: Native complex I, open2 手法: EM (単粒子) / 解像度: 3.3 Å 11259 6zkr EMDB-11259, PDB-6zkr: Native complex I, open3 手法: EM (単粒子) / 解像度: 3.5 Å 11260 6zks EMDB-11260, PDB-6zks: Deactive complex I, open1 手法: EM (単粒子) / 解像度: 3.1 Å 11261 6zkt EMDB-11261, PDB-6zkt: Deactive complex I, open2 手法: EM (単粒子) / 解像度: 2.8 Å 11262 6zku EMDB-11262, PDB-6zku: Deactive complex I, open3 手法: EM (単粒子) / 解像度: 3.0 Å 11263 6zkv EMDB-11263, PDB-6zkv: Deactive complex I, open4 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-15216: Complex I inhibited by rotenone open3 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-NAI: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / NADH ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-K: Unknown entry / カリウムカチオン ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-ZN: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-HOH: WATER ChemComp-DCQ: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione / デシルユビキノン ChemComp-AMP: ADENOSINE MONOPHOSPHATE / AMP / AMPYM ChemComp-MYR: MYRISTIC ACID / ミリスチン酸 ChemComp-970: (2R,6aS,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahydrofuro[2',3':7,8][1]benzopyrano[2,3-c][1]benzopyran-6(6aH)-one / ロテノン
由来	ovis aries (ヒツジ) Sheep (ヒツジ)
キーワード	ELECTRON TRANSPORT / complex / respiration / NADH / proton pump / mitochondria / iron-sulphur cluster / oxidoreductase / membrane protein