EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural visualization of transcription activated by a multidrug-sensing MerR family regulator.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 2702, Year 2021
掲載日	2021年5月11日
著者	Yang Yang / Chang Liu / Wei Zhou / Wei Shi / Ming Chen / Baoyue Zhang / David G Schatz / Yangbo Hu / Bin Liu /
PubMed 要旨	Bacterial RNA polymerase (RNAP) holoenzyme initiates transcription by recognizing the conserved -35 and -10 promoter elements that are optimally separated by a 17-bp spacer. The MerR family of ...Bacterial RNA polymerase (RNAP) holoenzyme initiates transcription by recognizing the conserved -35 and -10 promoter elements that are optimally separated by a 17-bp spacer. The MerR family of transcriptional regulators activate suboptimal 19-20 bp spacer promoters in response to myriad cellular signals, ranging from heavy metals to drug-like compounds. The regulation of transcription by MerR family regulators is not fully understood. Here we report one crystal structure of a multidrug-sensing MerR family regulator EcmrR and nine cryo-electron microscopy structures that capture the EcmrR-dependent transcription process from promoter opening to initial transcription to RNA elongation. These structures reveal that EcmrR is a dual ligand-binding factor that reshapes the suboptimal 19-bp spacer DNA to enable optimal promoter recognition, sustains promoter remodeling to stabilize initial transcribing complexes, and finally dissociates from the promoter to reverse DNA remodeling and facilitate the transition to elongation. Our findings yield a comprehensive model for transcription regulation by MerR family factors and provide insights into the transition from transcription initiation to elongation.
リンク	Nat Commun / PubMed:33976201 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.4 - 3.3 Å
構造データ	22234 6xl5 EMDB-22234, PDB-6xl5: Cryo-EM structure of EcmrR-RNAP-promoter open complex (EcmrR-RPo) 手法: EM (単粒子) / 解像度: 2.5 Å 22235 6xl6 EMDB-22235, PDB-6xl6: Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPo 手法: EM (単粒子) / 解像度: 3.0 Å 22236 6xl9 EMDB-22236, PDB-6xl9: Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA transcript (EcmrR-RPitc-3nt) 手法: EM (単粒子) / 解像度: 2.5 Å 22237 6xla EMDB-22237, PDB-6xla: Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPitc-3nt 手法: EM (単粒子) / 解像度: 3.1 Å 22245 6xlj EMDB-22245, PDB-6xlj: Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 4-nt RNA transcript (EcmrR-RPitc-4nt) 手法: EM (単粒子) / 解像度: 2.7 Å 22246 6xlk EMDB-22246, PDB-6xlk: Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPitc-4nt 手法: EM (単粒子) / 解像度: 3.3 Å 22247 6xll EMDB-22247, PDB-6xll: Cryo-EM structure of E. coli RNAP-promoter initial transcribing complex with 5-nt RNA transcript (RPitc-5nt) 手法: EM (単粒子) / 解像度: 2.7 Å 22248 6xlm EMDB-22248, PDB-6xlm: Cryo-EM structure of E.coli RNAP-DNA elongation complex 1 (RDe1) in EcmrR-dependent transcription 手法: EM (単粒子) / 解像度: 3.2 Å 22249 6xln EMDB-22249, PDB-6xln: Cryo-EM structure of E. coli RNAP-DNA elongation complex 2 (RDe2) in EcmrR-dependent transcription 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-23291: Cryo-EM map of EcmrR-RNAP-promoter open complex (EcmrR-RPo) with clear NCR-EcmrR NTD interface density 手法: EM (単粒子) / 解像度: 2.9 Å PDB-6wl5: Crystal structure of EcmrR C-terminal domain 手法: X-RAY DIFFRACTION / 解像度: 1.4 Å
化合物	ChemComp-16A: CETYL-TRIMETHYL-AMMONIUM / セチルトリメチルアンモニウム ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-CL: Unknown entry / クロリド ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル ChemComp-HOH: WATER ChemComp-1N7: CHAPSO / CHAPSO / 可溶化剤YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-118*: TETRAPHENYLANTIMONIUM ION / テトラフェニルスチボニウム
由来	escherichia coli o157:h7 (大腸菌) escherichia coli (大腸菌)
キーワード	TRANSCRIPTION / Transcriptional factor / TRANSFERASE/DNA / TRANSFERASE-DNA complex / promoter / multidrug recognition / TRANSCRIPTION TRANSFERASE-DNA complex / promoter escape