EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies.
ジャーナル・号・ページ	Cell, Vol. 182, Issue 4, Page 828-842.e16, Year 2020
掲載日	2020年8月20日
著者	Christopher O Barnes / Anthony P West / Kathryn E Huey-Tubman / Magnus A G Hoffmann / Naima G Sharaf / Pauline R Hoffman / Nicholas Koranda / Harry B Gristick / Christian Gaebler / Frauke Muecksch / Julio C Cetrulo Lorenzi / Shlomo Finkin / Thomas Hägglöf / Arlene Hurley / Katrina G Millard / Yiska Weisblum / Fabian Schmidt / Theodora Hatziioannou / Paul D Bieniasz / Marina Caskey / Davide F Robbiani / Michel C Nussenzweig / Pamela J Bjorkman /
PubMed 要旨	Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID- ...Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, recognition of alpha- and beta-coronaviruses, and contributions of avidity to increased binding/neutralization of IgGs over Fabs. Using electron microscopy, we examined specificities of polyclonal plasma Fabs, revealing recognition of both S1 and RBD epitopes on SARS-CoV-2 spike. Moreover, a 3.4 Å cryo-electron microscopy (cryo-EM) structure of a neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based on these structures suggested different potentials for inter-spike crosslinking by IgGs on viruses, and characterized IgGs would not be affected by identified SARS-CoV-2 spike mutations. Overall, our studies structurally define a recurrent anti-SARS-CoV-2 antibody class derived from VH3-53/VH3-66 and similarity to a SARS-CoV VH3-30 antibody, providing criteria for evaluating vaccine-elicited antibodies.
リンク	Cell / PubMed:32645326 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.8 - 21.0 Å
構造データ	EMDB-22124: SARS-CoV-2 S 2P negative-stain EM 手法: EM (単粒子) / 解像度: 16.72 Å EMDB-22125: SARS-CoV-2 S 2P trimer complexed with COV57 polyclonal Fabs 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-22126: SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from recovered COVID-19 individual COV21 手法: EM (単粒子) / 解像度: 21.0 Å 22127 6xcm EMDB-22127, PDB-6xcm: Structure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment (state 1) 手法: EM (単粒子) / 解像度: 3.42 Å 22128 6xcn EMDB-22128, PDB-6xcn: Structure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment (state 2) 手法: EM (単粒子) / 解像度: 3.66 Å PDB-6xca: Crystal structure of an anti-SARS-CoV-2 human neutralizing antibody Fab fragment, C105 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-HOH: WATER / 水 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM (免疫系) / Antibody (抗体) / SARS-CoV-2 (SARSコロナウイルス2) / SARS2 / Coronavirus (オルトコロナウイルス亜科) / Neutralizing (中和抗体) / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / Spike glycoprotein (スパイクタンパク質) / COVID-19 (新型コロナウイルス感染症 (2019年)) / monoclonal neutralizing antibody / Fabs / nsEMPEM / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性)