+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-22125 | |||||||||
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Title | SARS-CoV-2 S 2P trimer complexed with COV57 polyclonal Fabs | |||||||||
Map data | Negative-stain EM of SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from recovered COVID-19 individual, COV57 | |||||||||
Sample |
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Function / homology | Function and homology information Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / symbiont-mediated suppression of host innate immune response / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | Severe acute respiratory syndrome coronavirus 2 / Homo sapiens (human) | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 21.0 Å | |||||||||
Authors | Barnes CO / Bjorkman PJ | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Cell / Year: 2020 Title: Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies. Authors: Christopher O Barnes / Anthony P West / Kathryn E Huey-Tubman / Magnus A G Hoffmann / Naima G Sharaf / Pauline R Hoffman / Nicholas Koranda / Harry B Gristick / Christian Gaebler / Frauke ...Authors: Christopher O Barnes / Anthony P West / Kathryn E Huey-Tubman / Magnus A G Hoffmann / Naima G Sharaf / Pauline R Hoffman / Nicholas Koranda / Harry B Gristick / Christian Gaebler / Frauke Muecksch / Julio C Cetrulo Lorenzi / Shlomo Finkin / Thomas Hägglöf / Arlene Hurley / Katrina G Millard / Yiska Weisblum / Fabian Schmidt / Theodora Hatziioannou / Paul D Bieniasz / Marina Caskey / Davide F Robbiani / Michel C Nussenzweig / Pamela J Bjorkman / Abstract: Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID- ...Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of the trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, recognition of alpha- and beta-coronaviruses, and contributions of avidity to increased binding/neutralization of IgGs over Fabs. Using electron microscopy, we examined specificities of polyclonal plasma Fabs, revealing recognition of both S1 and RBD epitopes on SARS-CoV-2 spike. Moreover, a 3.4 Å cryo-electron microscopy (cryo-EM) structure of a neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based on these structures suggested different potentials for inter-spike crosslinking by IgGs on viruses, and characterized IgGs would not be affected by identified SARS-CoV-2 spike mutations. Overall, our studies structurally define a recurrent anti-SARS-CoV-2 antibody class derived from VH3-53/VH3-66 and similarity to a SARS-CoV VH3-30 antibody, providing criteria for evaluating vaccine-elicited antibodies. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_22125.map.gz | 31.5 MB | EMDB map data format | |
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Header (meta data) | emd-22125-v30.xml emd-22125.xml | 12 KB 12 KB | Display Display | EMDB header |
Images | emd_22125.png | 18.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-22125 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-22125 | HTTPS FTP |
-Validation report
Summary document | emd_22125_validation.pdf.gz | 78.7 KB | Display | EMDB validaton report |
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Full document | emd_22125_full_validation.pdf.gz | 77.9 KB | Display | |
Data in XML | emd_22125_validation.xml.gz | 494 B | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-22125 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-22125 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_22125.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Negative-stain EM of SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from recovered COVID-19 individual, COV57 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from COV57 ...
Entire | Name: SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from COV57 individual |
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Components |
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-Supramolecule #1: SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from COV57 ...
Supramolecule | Name: SARS-CoV-2 S 2P trimer complexed with polyclonal Fabs from COV57 individual type: complex / ID: 1 / Parent: 0 |
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Molecular weight | Experimental: 600 KDa |
-Supramolecule #2: SARS-CoV-2 spike glycoprotein 2P trimer
Supramolecule | Name: SARS-CoV-2 spike glycoprotein 2P trimer / type: complex / ID: 2 / Parent: 1 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant cell: Expi293F / Recombinant plasmid: pCAGGS |
Molecular weight | Experimental: 585 KDa |
-Supramolecule #3: COV57 polyclonal Fab fragment
Supramolecule | Name: COV57 polyclonal Fab fragment / type: complex / ID: 3 / Parent: 1 Details: COV57 polyclonal Fab fragments were generated by proteolytic cleavage of plasma-purified IgG antibodies. |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Experimental: 50 KDa |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.05 mg/mL |
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Buffer | pH: 8 |
Staining | Type: NEGATIVE / Material: Uranyl Formate / Details: 1% uranyl formate staining |
Grid | Details: unspecified |
Details | Monodisperse sample taken after size-exclusion chromatography |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average exposure time: 2.0 sec. / Average electron dose: 12.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |