+検索条件
-Structure paper
タイトル | mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding. |
---|---|
ジャーナル・号・ページ | Elife, Vol. 9, Year 2020 |
掲載日 | 2020年5月19日 |
著者 | Chen Bao / Sarah Loerch / Clarence Ling / Andrei A Korostelev / Nikolaus Grigorieff / Dmitri N Ermolenko / |
PubMed 要旨 | Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via ...Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from mRNA and the transcript of Human Immunodeficiency Virus (HIV) perturb translation elongation. We find that upon encountering the ribosome, the stem-loops strongly inhibit A-site tRNA binding and ribosome intersubunit rotation that accompanies translation elongation. Electron cryo-microscopy (cryo-EM) reveals that the HIV stem-loop docks into the A site of the ribosome. Our results suggest that mRNA stem-loops can transiently escape the ribosome helicase by binding to the A site. Thus, the stem-loops can modulate gene expression by sterically hindering tRNA binding and inhibiting translation elongation. |
リンク | Elife / PubMed:32427100 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 3.4 Å |
構造データ | EMDB-21420, PDB-6vwl: EMDB-21421, PDB-6vwm: EMDB-21422, PDB-6vwn: |
化合物 | ChemComp-MG: |
由来 |
|
キーワード | RIBOSOME / HIV FSS / frameshifting |