EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Action of a minimal contractile bactericidal nanomachine.
ジャーナル・号・ページ	Nature, Vol. 580, Issue 7805, Page 658-662, Year 2020
掲載日	2020年4月15日
著者	Peng Ge / Dean Scholl / Nikolai S Prokhorov / Jaycob Avaylon / Mikhail M Shneider / Christopher Browning / Sergey A Buth / Michel Plattner / Urmi Chakraborty / Ke Ding / Petr G Leiman / Jeff F Miller / Z Hong Zhou /
PubMed 要旨	R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded ...R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
リンク	Nature / PubMed:32350467 / PubMed Central
手法	EM (らせん対称) / EM (単粒子) / X線回折
解像度	2.102 - 4.0 Å
構造データ	20526 6pyt EMDB-20526, PDB-6pyt: CryoEM Structure of Pyocin R2 - precontracted - trunk 手法: EM (らせん対称) / 解像度: 2.9 Å 20643 6u5b EMDB-20643, PDB-6u5b: CryoEM Structure of Pyocin R2 - precontracted - baseplate 手法: EM (単粒子) / 解像度: 3.5 Å 20644 6u5f EMDB-20644, PDB-6u5f: CryoEM Structure of Pyocin R2 - precontracted - collar 手法: EM (単粒子) / 解像度: 3.8 Å 20646 6u5h EMDB-20646, PDB-6u5h: CryoEM Structure of Pyocin R2 - precontracted - hub 手法: EM (単粒子) / 解像度: 4.0 Å 20647 6u5j EMDB-20647, PDB-6u5j: CryoEM Structure of Pyocin R2 - postcontracted - collar 手法: EM (単粒子) / 解像度: 3.5 Å 20648 6u5k EMDB-20648, PDB-6u5k: CryoEM Structure of Pyocin R2 - postcontracted - baseplate 手法: EM (単粒子) / 解像度: 3.5 Å PDB-5ces: C-terminal domain of the R-type pyocin baseplate protein PA0618 手法: X-RAY DIFFRACTION / 解像度: 2.102 Å
化合物	ChemComp-HOH: WATER
由来	pseudomonas aeruginosa pao1 (緑膿菌) pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (緑膿菌)
キーワード	STRUCTURAL PROTEIN / gpJ / gp6 / ANTIMICROBIAL PROTEIN / bacteriocin / pyocin / helix / VIRAL PROTEIN / UNKNOWN FUNCTION