EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of ribosomes from an aminoglycoside-resistant clinical isolate.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 44, Page 22275-22281, Year 2019
掲載日	2019年10月29日
著者	Yehuda Halfon / Alicia Jimenez-Fernandez / Ruggero La Rosa / Rocio Espinosa Portero / Helle Krogh Johansen / Donna Matzov / Zohar Eyal / Anat Bashan / Ella Zimmerman / Matthew Belousoff / Søren Molin / Ada Yonath /
PubMed 要旨	Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a ...Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a major target for clinically useful antibiotics. We report here the cryo-electron microscopy structures of the ribosome of a pathogenic aminoglycoside (AG)-resistant strain, as well as of a nonresistance strain isolated from a cystic fibrosis patient. The structural studies disclosed defective ribosome complex formation due to a conformational change of rRNA helix H69, an essential intersubunit bridge, and a secondary binding site of the AGs. In addition, a stable conformation of nucleotides A1486 and A1487, pointing into helix h44, is created compared to a non-AG-bound ribosome. We suggest that altering the conformations of ribosomal protein uL6 and rRNA helix H69, which interact with initiation-factor IF2, interferes with proper protein synthesis initiation.
リンク	Proc Natl Acad Sci U S A / PubMed:31611393 / PubMed Central
手法	EM (単粒子)
解像度	2.82 - 3.6 Å
構造データ	10280 6spb EMDB-10280, PDB-6spb: Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 手法: EM (単粒子) / 解像度: 2.82 Å 10281 6spc EMDB-10281, PDB-6spc: Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate 手法: EM (単粒子) / 解像度: 2.95 Å 10282 6spd EMDB-10282, PDB-6spd: Pseudomonas aeruginosa 50s ribosome from a clinical isolate 手法: EM (単粒子) / 解像度: 3.28 Å 10283 6spe EMDB-10283, PDB-6spe: Pseudomonas aeruginosa 30s ribosome from a clinical isolate 手法: EM (単粒子) / 解像度: 3.6 Å 10284 6spf EMDB-10284, PDB-6spf: Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate 手法: EM (単粒子) / 解像度: 2.89 Å 10285 6spg EMDB-10285, PDB-6spg: Pseudomonas aeruginosa 70s ribosome from a clinical isolate 手法: EM (単粒子) / 解像度: 3.34 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	Pseudomonas aeruginos (緑膿菌) pseudomonas aeruginosa (緑膿菌)
キーワード	RIBOSOME / Pseudomonas aeruginosa / mutation