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-Structure paper
タイトル | Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56γ. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 5043, Year 2020 |
掲載日 | 2020年10月7日 |
![]() | Michał S Barski / Jordan J Minnell / Zuzana Hodakova / Valerie E Pye / Andrea Nans / Peter Cherepanov / Goedele N Maertens / ![]() |
PubMed 要旨 | Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor ...Human T-cell lymphotropic virus type 1 (HTLV-1) is a deltaretrovirus and the most oncogenic pathogen. Many of the ~20 million HTLV-1 infected people will develop severe leukaemia or an ALS-like motor disease, unless a therapy becomes available. A key step in the establishment of infection is the integration of viral genetic material into the host genome, catalysed by the retroviral integrase (IN) enzyme. Here, we use X-ray crystallography and single-particle cryo-electron microscopy to determine the structure of the functional deltaretroviral IN assembled on viral DNA ends and bound to the B56γ subunit of its human host factor, protein phosphatase 2 A. The structure reveals a tetrameric IN assembly bound to two molecules of the phosphatase via a conserved short linear motif. Insight into the deltaretroviral intasome and its interaction with the host will be crucial for understanding the pattern of integration events in infected individuals and therefore bears important clinical implications. |
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手法 | X線回折 / EM (単粒子) |
解像度 | 1.8 - 3.34 Å |
構造データ | ![]() PDB-6qbt: ![]() PDB-6qbv: ![]() PDB-6qbw: ![]() PDB-6tju: ![]() PDB-6toq: ![]() PDB-7pel: |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-HOH: ![]() ChemComp-CA: ![]() ChemComp-ZN: |
由来 |
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![]() | TRANSFERASE / retrovirus / deltaretrovirus / integration / strand-transfer / nucleotidyltransferase / HTLV-1 / integrase / DNA-binding / SIGNALING PROTEIN / Phosphatase / complex / SLiM / dephosphorylation / cell signalling / motif mimicry / VIRAL PROTEIN / PP2A / B56 / molecular mimicry / host factor |