EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 2393, Year 2019
掲載日	2019年6月3日
著者	Alexandrea N Rizo / JiaBei Lin / Stephanie N Gates / Eric Tse / Stephen M Bart / Laura M Castellano / Frank DiMaio / James Shorter / Daniel R Southworth /
PubMed 要旨	Bacterial ClpB and yeast Hsp104 are homologous Hsp100 protein disaggregases that serve critical functions in proteostasis by solubilizing protein aggregates. Two AAA+ nucleotide binding domains (NBDs) ...Bacterial ClpB and yeast Hsp104 are homologous Hsp100 protein disaggregases that serve critical functions in proteostasis by solubilizing protein aggregates. Two AAA+ nucleotide binding domains (NBDs) power polypeptide translocation through a central channel comprised of a hexameric spiral of protomers that contact substrate via conserved pore-loop interactions. Here we report cryo-EM structures of a hyperactive ClpB variant bound to the model substrate, casein in the presence of slowly hydrolysable ATPγS, which reveal the translocation mechanism. Distinct substrate-gripping interactions are identified for NBD1 and NBD2 pore loops. A trimer of N-terminal domains define a channel entrance that binds the polypeptide substrate adjacent to the topmost NBD1 contact. NBD conformations at the seam interface reveal how ATP hydrolysis-driven substrate disengagement and re-binding are precisely tuned to drive a directional, stepwise translocation cycle.
リンク	Nat Commun / PubMed:31160557 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.1 Å
構造データ	20004 6oax EMDB-20004: CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, pre-state PDB-6oax: Structure of the hyperactive ClpB mutant K476C, bound to casein, pre-state 手法: EM (単粒子) / 解像度: 2.9 Å 20005 6oay EMDB-20005: CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, post-state PDB-6oay: Structure of the hyperactive ClpB mutant K476C, bound to casein, post-state 手法: EM (単粒子) / 解像度: 3.3 Å 20049 6og1 EMDB-20049: CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, pre-state PDB-6og1: Focus classification structure of the hyperactive ClpB mutant K476C, bound to casein, pre-state 手法: EM (単粒子) / 解像度: 3.3 Å 20050 6og2 EMDB-20050: CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, post-state PDB-6og2: Focus classification structure of the hyperactive ClpB mutant K476C, bound to casein, post-state 手法: EM (単粒子) / 解像度: 4.1 Å 20051 6og3 EMDB-20051: CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer PDB-6og3: Focus classification structure of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	escherichia coli k-12 (大腸菌) bos taurus (ウシ)
キーワード	CHAPERONE (シャペロン) / disaggregase / CLPB / AAA+