EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of vacuolating cytotoxin A oligomeric assemblies at near-atomic resolution.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 14, Page 6800-6805, Year 2019
掲載日	2019年4月2日
著者	Kaiming Zhang / Huawei Zhang / Shanshan Li / Grigore D Pintilie / Tung-Chung Mou / Yuanzhu Gao / Qinfen Zhang / Henry van den Bedem / Michael F Schmid / Shannon Wing Ngor Au / Wah Chiu /
PubMed 要旨	Human gastric pathogen () is the primary risk factor for gastric cancer and is one of the most prevalent carcinogenic infectious agents. Vacuolating cytotoxin A (VacA) is a key virulence factor ...Human gastric pathogen () is the primary risk factor for gastric cancer and is one of the most prevalent carcinogenic infectious agents. Vacuolating cytotoxin A (VacA) is a key virulence factor secreted by and induces multiple cellular responses. Although structural and functional studies of VacA have been extensively performed, the high-resolution structure of a full-length VacA protomer and the molecular basis of its oligomerization are still unknown. Here, we use cryoelectron microscopy to resolve 10 structures of VacA assemblies, including monolayer (hexamer and heptamer) and bilayer (dodecamer, tridecamer, and tetradecamer) oligomers. The models of the 88-kDa full-length VacA protomer derived from the near-atomic resolution maps are highly conserved among different oligomers and show a continuous right-handed β-helix made up of two domains with extensive domain-domain interactions. The specific interactions between adjacent protomers in the same layer stabilizing the oligomers are well resolved. For double-layer oligomers, we found short- and/or long-range hydrophobic interactions between protomers across the two layers. Our structures and other previous observations lead to a mechanistic model wherein VacA hexamer would correspond to the prepore-forming state, and the N-terminal region of VacA responsible for the membrane insertion would undergo a large conformational change to bring the hydrophobic transmembrane region to the center of the oligomer for the membrane channel formation.
リンク	Proc Natl Acad Sci U S A / PubMed:30894496 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 9.9 Å
構造データ	0542 6nyf EMDB-0542, PDB-6nyf: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 1 (OA-1) 手法: EM (単粒子) / 解像度: 3.2 Å 0543 6nyg EMDB-0543, PDB-6nyg: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2a (OA-2a) 手法: EM (単粒子) / 解像度: 3.9 Å 0544 6nyj EMDB-0544, PDB-6nyj: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2b (OA-2b) 手法: EM (単粒子) / 解像度: 3.2 Å 0545 6nyl EMDB-0545, PDB-6nyl: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2c (OA-2c) 手法: EM (単粒子) / 解像度: 3.7 Å 0546 6nym EMDB-0546, PDB-6nym: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2d (OA-2d) 手法: EM (単粒子) / 解像度: 3.6 Å 0547 6nyn EMDB-0547, PDB-6nyn: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2e (OA-2e) 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-0548: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 2f (OA-2f) 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-0549: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 3 (OA-3) 手法: EM (単粒子) / 解像度: 9.9 Å EMDB-0550: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 4 (OA-4) 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-0551: Helicobacter pylori Vacuolating Cytotoxin A Oligomeric Assembly 5 (OA-5) 手法: EM (単粒子) / 解像度: 7.4 Å
由来	helicobacter pylori (ピロリ菌)
キーワード	TOXIN / Helicobacter pylori / vacuolating cytotoxin A / pore-forming toxin