EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanisms for Zinc and Proton Inhibition of the GluN1/GluN2A NMDA Receptor.
ジャーナル・号・ページ	Cell, Vol. 175, Issue 6, Page 1520-1532.e15, Year 2018
掲載日	2018年11月29日
著者	Farzad Jalali-Yazdi / Sandipan Chowdhury / Craig Yoshioka / Eric Gouaux /
PubMed 要旨	N-methyl-D-aspartate receptors (NMDARs) play essential roles in memory formation, neuronal plasticity, and brain development, with their dysfunction linked to a range of disorders from ischemia to ...N-methyl-D-aspartate receptors (NMDARs) play essential roles in memory formation, neuronal plasticity, and brain development, with their dysfunction linked to a range of disorders from ischemia to schizophrenia. Zinc and pH are physiological allosteric modulators of NMDARs, with GluN2A-containing receptors inhibited by nanomolar concentrations of divalent zinc and by excursions to low pH. Despite the widespread importance of zinc and proton modulation of NMDARs, the molecular mechanism by which these ions modulate receptor activity has proven elusive. Here, we use cryoelectron microscopy to elucidate the structure of the GluN1/GluN2A NMDAR in a large ensemble of conformations under a range of physiologically relevant zinc and proton concentrations. We show how zinc binding to the amino terminal domain elicits structural changes that are transduced though the ligand-binding domain and result in constriction of the ion channel gate.
リンク	Cell / PubMed:30500536 / PubMed Central
手法	EM (単粒子)
解像度	4.71 - 16.5 Å
構造データ	9147 6mm9 EMDB-9147, PDB-6mm9: Diheteromeric NMDA receptor GluN1/GluN2A in the '1-Knuckle' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 6.1 手法: EM (単粒子) / 解像度: 5.97 Å 9148 6mma EMDB-9148, PDB-6mma: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Extended' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 6.1 手法: EM (単粒子) / 解像度: 6.31 Å 9149 6mmb EMDB-9149, PDB-6mmb: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Super-Splayed' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 6.1 手法: EM (単粒子) / 解像度: 12.7 Å 9150 6mmg EMDB-9150, PDB-6mmg: Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar EDTA, and at pH 7.4 手法: EM (単粒子) / 解像度: 6.23 Å 9151 6mmh EMDB-9151, PDB-6mmh: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Extended-2' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 8.21 Å 9152 6mmi EMDB-9152, PDB-6mmi: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Splayed-Open' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 8.93 Å 9153 6mmj EMDB-9153, PDB-6mmj: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Super-Splayed' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 16.5 Å 9154 6mmk EMDB-9154, PDB-6mmk: Diheteromeric NMDA receptor GluN1/GluN2A in the '1-Knuckle' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 6.08 Å 9155 6mml EMDB-9155, PDB-6mml: Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 7.14 Å 9156 6mmm EMDB-9156, PDB-6mmm: Diheteromeric NMDA receptor GluN1/GluN2A in the 'Extended-1' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 6.84 Å 9157 6mmn EMDB-9157, PDB-6mmn: Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 8.0 手法: EM (単粒子) / 解像度: 7.51 Å 9158 6mmp EMDB-9158, PDB-6mmp: Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 0.1 millimolar EDTA, and at pH 8.0 手法: EM (単粒子) / 解像度: 6.88 Å 9159 6mmr EMDB-9159, PDB-6mmr: Diheteromeric NMDA receptor GluN1/GluN2A in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar zinc chloride, 3 millimolar EDTA, and at pH 7.4 手法: EM (単粒子) / 解像度: 5.13 Å 9160 6mms EMDB-9160, PDB-6mms: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 millimolar EDTA, and at pH 7.4 手法: EM (単粒子) / 解像度: 5.38 Å 9161 6mmt EMDB-9161, PDB-6mmt: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* in the '1-Knuckle' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 7.46 Å 9162 6mmu EMDB-9162, PDB-6mmu: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 5.3 Å 9163 6mmv EMDB-9163, PDB-6mmv: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* Extracellular Domain in the '2-Knuckle-Asymmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 4.71 Å 9164 6mmw EMDB-9164, PDB-6mmw: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* in the '2-Knuckle-Symmetric' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 6.2 Å 9165 6mmx EMDB-9165, PDB-6mmx: Triheteromeric NMDA receptor GluN1/GluN2A/GluN2A* in the 'Extended' conformation, in complex with glycine and glutamate, in the presence of 1 micromolar zinc chloride, and at pH 7.4 手法: EM (単粒子) / 解像度: 6.99 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	rattus norvegicus (ドブネズミ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Ligand-gated Ion Channel (リガンド依存性イオンチャネル) / NMDA Receptor (NMDA型グルタミン酸受容体) / ionotropic Glutamate Receptors / membrane protein (膜タンパク質)