EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.
ジャーナル・号・ページ	Cell Host Microbe, Vol. 27, Issue 2, Page 249-261.e5, Year 2020
掲載日	2020年2月12日
著者	Maozhou He / Longfa Xu / Qingbing Zheng / Rui Zhu / Zhichao Yin / Zhenghui Zha / Yu Lin / Lisheng Yang / Yang Huang / Xiangzhong Ye / Shuxuan Li / Wangheng Hou / Yangtao Wu / Jinle Han / Dongxiao Liu / Zekai Li / Zhenqin Chen / Hai Yu / Yuqiong Que / Yingbin Wang / Xiaodong Yan / Jun Zhang / Ying Gu / Z Hong Zhou / Tong Cheng / Shaowei Li / Ningshao Xia /
PubMed 要旨	Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific ...Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms-the mature virion, A-particle, and empty particle-and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.
リンク	Cell Host Microbe / PubMed:32027857 / PubMed Central
手法	EM (単粒子)
解像度	2.65 - 3.78 Å
構造データ	0887 6lha EMDB-0887, PDB-6lha: The cryo-EM structure of coxsackievirus A16 mature virion 手法: EM (単粒子) / 解像度: 3.56 Å 0888 6lhb EMDB-0888, PDB-6lhb: The cryo-EM structure of coxsackievirus A16 A-particle 手法: EM (単粒子) / 解像度: 3.33 Å 0889 6lhc EMDB-0889, PDB-6lhc: The cryo-EM structure of coxsackievirus A16 empty particle 手法: EM (単粒子) / 解像度: 3.43 Å 0890 6lhk EMDB-0890, PDB-6lhk: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab 18A7 手法: EM (単粒子) / 解像度: 2.65 Å 0891 6lhl EMDB-0891, PDB-6lhl: The cryo-EM structure of coxsackievirus A16 A-particle in complex with Fab 18A7 手法: EM (単粒子) / 解像度: 3.07 Å 0892 6lho EMDB-0892, PDB-6lho: The cryo-EM structure of coxsackievirus A16 empty particle in complex with Fab 18A7 手法: EM (単粒子) / 解像度: 3.13 Å 0894 6lhp EMDB-0894, PDB-6lhp: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab 14B10 手法: EM (単粒子) / 解像度: 3.3 Å 0895 6lhq EMDB-0895, PDB-6lhq: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab NA9D7 手法: EM (単粒子) / 解像度: 3.06 Å 0897 6lht EMDB-0897, PDB-6lht: Localized reconstruction of coxsackievirus A16 mature virion in complex with Fab 18A7 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-0898: The cryo-EM structure of coxsackievirus A16 mature virion in complex with Fabs 18A7, 14B10 and NA9D7 手法: EM (単粒子) / 解像度: 3.78 Å
化合物	ChemComp-SPH: SPHINGOSINE / スフィンゴシン
由来	coxsackievirus a16 (コクサッキーウイルス) mus musculus (ハツカネズミ)
キーワード	VIRUS (ウイルス) / VIRAL PROTEIN (ウイルスタンパク質) / localized reconstruction