EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insight into RNA synthesis by influenza D polymerase.
ジャーナル・号・ページ	Nat Microbiol, Vol. 4, Issue 10, Page 1750-1759, Year 2019
掲載日	2019年6月17日
著者	Qi Peng / Yuqian Liu / Ruchao Peng / Min Wang / Wei Yang / Hao Song / Yuhai Chen / Sheng Liu / Min Han / Xinzheng Zhang / Peiyi Wang / Jinghua Yan / Buchang Zhang / Jianxun Qi / Tao Deng / George F Gao / Yi Shi /
PubMed 要旨	The influenza virus polymerase uses capped RNA primers to initiate transcription, and a combination of terminal and internal de novo initiations for the two-step replication process by binding the ...The influenza virus polymerase uses capped RNA primers to initiate transcription, and a combination of terminal and internal de novo initiations for the two-step replication process by binding the conserved viral genomic RNA (vRNA) or complementary RNA (cRNA) promoter. Here, we determined the apo and promoter-bound influenza D polymerase structures using cryo-electron microscopy and found the polymerase has an evolutionarily conserved stable core structure with inherently flexible peripheral domains. Strikingly, two conformations (mode A and B) of the vRNA promoter were observed where the 3'-vRNA end can bind at two different sites, whereas the cRNA promoter only binds in the mode B conformation. Functional studies confirmed the critical role of the mode B conformation for vRNA synthesis via the intermediate cRNA but not for cRNA production, which is mainly regulated by the mode A conformation. Both conformations participate in the regulation of the transcription process. This work advances our understanding of the regulatory mechanisms for the synthesis of different RNA species by influenza virus polymerase and opens new opportunities for antiviral drug design.
リンク	Nat Microbiol / PubMed:31209309
手法	EM (単粒子)
解像度	3.4 - 4.6 Å
構造データ	9577 6kv5 EMDB-9577: structure of influenza D virus apo polymerase PDB-6kv5: Structure of influenza D virus apo polymerase 手法: EM (単粒子) / 解像度: 4.6 Å 9578 6kuk EMDB-9578, PDB-6kuk: Structure of influenza D virus polymerase bound to vRNA promoter in mode A conformation (class A1) 手法: EM (単粒子) / 解像度: 3.9 Å 9579 6kup EMDB-9579, PDB-6kup: Structure of influenza D virus polymerase bound to vRNA promoter in Mode A conformation(Class A2) 手法: EM (単粒子) / 解像度: 4.3 Å 9580 6kut EMDB-9580: structure of influenza D virus polymerase bound to vRNA promoter in Mode B conformation (Class B2) PDB-6kut: Structure of influenza D virus polymerase bound to vRNA promoter in Mode B conformation (Class B2) 手法: EM (単粒子) / 解像度: 4.1 Å 9581 6kur EMDB-9581, PDB-6kur: Structure of influenza D virus polymerase bound to vRNA promoter in Mode B conformation (Class B1) 手法: EM (単粒子) / 解像度: 3.7 Å 9582 6kuu EMDB-9582, PDB-6kuu: Structure of influenza D virus polymerase bound to vRNA promoter in Mode B conformation (Class B3) 手法: EM (単粒子) / 解像度: 4.0 Å 9887 6kuj EMDB-9887, PDB-6kuj: Structure of influenza D virus polymerase bound to cRNA promoter in class 1 手法: EM (単粒子) / 解像度: 3.44 Å 9888 6kuv EMDB-9888, PDB-6kuv: Structure of influenza D virus polymerase bound to cRNA promoter in class 2 手法: EM (単粒子) / 解像度: 4.1 Å
由来	Thogotovirus (トゴトウイルス属) influenza d virus (d/swine/oklahoma/1334/2011) (インフルエンザウイルス) Influenza D virus (インフルエンザウイルス) synthetic construct (人工物)
キーワード	VIRAL PROTEIN/RNA (ウイルス性) / influenza D virus (D型インフルエンザウイルス) / polymerase (ポリメラーゼ) / cryo-EM (低温電子顕微鏡法) / VIRAL PROTEIN-RNA complex (ウイルス性) / RNA BINDING PROTEIN (RNA結合タンパク質) / VIRAL PROTEIN (ウイルスタンパク質)