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-Structure paper
タイトル | Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 25, Issue 2, Page 135-138, Year 2018 |
掲載日 | 2018年1月22日 |
![]() | Marcello Clerici / Marco Faini / Lena M Muckenfuss / Ruedi Aebersold / Martin Jinek / ![]() |
PubMed 要旨 | Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and ...Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation. |
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手法 | EM (単粒子) |
解像度 | 3.07 Å |
構造データ | |
化合物 | ![]() ChemComp-ZN: |
由来 |
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![]() | RNA BINDING PROTEIN / 3' pre-mRNA processing / polyadenylation / CPSF / beta propeller / AAUAAA |