EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for the regulation of inositol trisphosphate receptors by Ca and IP.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 25, Issue 8, Page 660-668, Year 2018
掲載日	2018年7月16日
著者	Navid Paknejad / Richard K Hite /
PubMed 要旨	Inositol trisphosphate receptors (IPRs) are ubiquitous Ca-permeable channels that mediate release of Ca from the endoplasmic reticulum, thereby regulating numerous processes including cell division, ...Inositol trisphosphate receptors (IPRs) are ubiquitous Ca-permeable channels that mediate release of Ca from the endoplasmic reticulum, thereby regulating numerous processes including cell division, cell death, differentiation and fertilization. IPRs are jointly activated by inositol trisphosphate (IP) and their permeant ion, Ca. At high concentrations, however, Ca inhibits activity, ensuring precise spatiotemporal control over intracellular Ca. Despite extensive characterization of IPR, the mechanisms through which these molecules control channel gating have remained elusive. Here, we present structures of full-length human type 3 IPRs in ligand-bound and ligand-free states. Multiple IP-bound structures demonstrate that the large cytoplasmic domain provides a platform for propagation of long-range conformational changes to the ion-conduction gate. Structures in the presence of Ca reveal two Ca-binding sites that induce the disruption of numerous interactions between subunits, thereby inhibiting IPR. These structures thus provide a mechanistic basis for beginning to understand the regulation of IPR.
リンク	Nat Struct Mol Biol / PubMed:30013099 / PubMed Central
手法	EM (単粒子)
解像度	3.26 - 6.01 Å
構造データ	7978 6dqj EMDB-7978, PDB-6dqj: Human type 3 1,4,5-inositol trisphosphate receptor in a ligand-free state 手法: EM (単粒子) / 解像度: 3.49 Å EMDB-7979: Cytoplasmic domain of human type 3 1,4,5-inositol trisphosphate receptor in a ligand-free state 手法: EM (単粒子) / 解像度: 3.69 Å EMDB-7980: Human type 3 1,4,5-inositol trisphosphate receptor in a ligand-free state 手法: EM (単粒子) / 解像度: 3.69 Å 7981 6dqn EMDB-7981, PDB-6dqn: Class 1 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.33 Å EMDB-7982: Cytoplasmic domain of class 1 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.26 Å 7983 6dqs EMDB-7983, PDB-6dqs: Class 3 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.12 Å 7984 6dqv EMDB-7984, PDB-6dqv: Class 2 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.82 Å EMDB-7985: Cytoplasmic domain of class 2 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.94 Å 7986 6dqz EMDB-7986, PDB-6dqz: Class 4 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 6.01 Å 7987 6dr0 EMDB-7987, PDB-6dr0: Class 5 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.47 Å 7988 6dr2 EMDB-7988, PDB-6dr2: Ca2+-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.33 Å EMDB-7989: Cytoplasmic domain of Ca2+-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.69 Å EMDB-7990: Transmembrane domain of Ca2+-bound human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.22 Å 7991 6dra EMDB-7991, PDB-6dra: Low IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.96 Å EMDB-7992: Cytoplasmic domain of low IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-7993: Transmembrane domain of low IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.8 Å 7994 6drc EMDB-7994, PDB-6drc: High IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.92 Å EMDB-7995: Transmembrane domain of high IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.66 Å EMDB-7996: Cytoplasmic domain of high IP3 Ca2+ human type 3 1,4,5-inositol trisphosphate receptor 手法: EM (単粒子) / 解像度: 3.78 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-I3P: D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE / イノシト-ル1,4,5-トリスりん酸 ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト)
キーワード	METAL TRANSPORT / Ion channel / Calcium channel