EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transposon molecular domestication and the evolution of the RAG recombinase.
ジャーナル・号・ページ	Nature, Vol. 569, Issue 7754, Page 79-84, Year 2019
掲載日	2019年4月10日
著者	Yuhang Zhang / Tat Cheung Cheng / Guangrui Huang / Qingyi Lu / Marius D Surleac / Jeffrey D Mandell / Pierre Pontarotti / Andrei J Petrescu / Anlong Xu / Yong Xiong / David G Schatz /
PubMed 要旨	Domestication of a transposon (a DNA sequence that can change its position in a genome) to give rise to the RAG1-RAG2 recombinase (RAG) and V(D)J recombination, which produces the diverse repertoire ...Domestication of a transposon (a DNA sequence that can change its position in a genome) to give rise to the RAG1-RAG2 recombinase (RAG) and V(D)J recombination, which produces the diverse repertoire of antibodies and T cell receptors, was a pivotal event in the evolution of the adaptive immune system of jawed vertebrates. The evolutionary adaptations that transformed the ancestral RAG transposase into a RAG recombinase with appropriately regulated DNA cleavage and transposition activities are not understood. Here, beginning with cryo-electron microscopy structures of the amphioxus ProtoRAG transposase (an evolutionary relative of RAG), we identify amino acid residues and domains the acquisition or loss of which underpins the propensity of RAG for coupled cleavage, its preference for asymmetric DNA substrates and its inability to perform transposition in cells. In particular, we identify two adaptations specific to jawed-vertebrates-arginine 848 in RAG1 and an acidic region in RAG2-that together suppress RAG-mediated transposition more than 1,000-fold. Our findings reveal a two-tiered mechanism for the suppression of RAG-mediated transposition, illuminate the evolution of V(D)J recombination and provide insight into the principles that govern the molecular domestication of transposons.
リンク	Nature / PubMed:30971819 / PubMed Central
手法	EM (単粒子)
解像度	4.3 - 5.3 Å
構造データ	EMDB-7043: BbRAG1L-3'TIR synaptic complex with intact DNA refined with C1 symmetry 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-7044: BbRAG1L- 3'TIR synaptic complex with intact DNA refined with C2 symmetry 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-7045: BbRAGL-3'TIR synaptic complex with nicked DNA refined with c1 symmetry 手法: EM (単粒子) / 解像度: 5.0 Å 7046 6b40 EMDB-7046, PDB-6b40: BbRAGL-3'TIR synaptic complex with nicked DNA refined with C2 symmetry 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ZN: Unknown entry
由来	branchiostoma belcheri (ナメクジウオ)
キーワード	RECOMBINATION / DNA transposase / DNA cut and paste transposition / DDE family RNase H fold DNA transposase