EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 45, Issue 18, Page 10884-10894, Year 2017
掲載日	2017年10月13日
著者	Kailu Yang / Jeng-Yih Chang / Zhicheng Cui / Xiaojun Li / Ran Meng / Lijun Duan / Jirapat Thongchol / Joanita Jakana / Christoph M Huwe / James C Sacchettini / Junjie Zhang /
PubMed 要旨	Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the ...Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23S rRNA, named H54a or the 'handle', switches interactions from with rRNA helix H68 and rProtein uL2 to with rProtein bS6, forming a new intersubunit bridge 'B9'. In Mtb 70S, bridge B9 is mostly maintained, leading to correlated motions among the handle, the L1 stalk and the small subunit in the rotated and non-rotated states. Two new protein densities were discovered near the decoding center and the peptidyl transferase center, respectively. These results provide a structural basis for studying translation in Mtb as well as developing new tuberculosis drugs.
リンク	Nucleic Acids Res / PubMed:28977617 / PubMed Central
手法	EM (単粒子)
解像度	3.7 - 7.1 Å
構造データ	8641 5v7q EMDB-8641: Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis PDB-5v7q: Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis bound with a potent linezolid analog 手法: EM (単粒子) / 解像度: 3.7 Å 8645 5v93 EMDB-8645, PDB-5v93: Cryo-EM structure of the 70S ribosome from Mycobacterium tuberculosis bound with Capreomycin 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-8646: Locally-refined cryo-EM structure of the small ribosomal subunit from Mycobacterium tuberculosis 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8647: Cryo-EM structure of the 70S ribosome from Mycobacterium smegmatis 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-8648: Focused classification of the 70S ribosome from Mycobacterium tuberculosis 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-8649: Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis at 1 mM magnesium concentration 手法: EM (単粒子) / 解像度: 6.0 Å
化合物	ChemComp-917: N-({(5S)-2-oxo-3-[4-(1,3-thiazol-5-yl)phenyl]-1,3-oxazolidin-5-yl}methyl)acetamide
由来	mycobacterium tuberculosis (結核菌) streptomyces puniceus (バクテリア) Mycobacterium smegmatis (バクテリア)
キーワード	RIBOSOME (リボソーム) / RNA dynamics / RNA complex (リボ核酸)