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-Structure paper
タイトル | The architecture of respiratory supercomplexes. |
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ジャーナル・号・ページ | Nature, Vol. 537, Issue 7622, Page 644-648, Year 2016 |
掲載日 | 2016年9月29日 |
著者 | James A Letts / Karol Fiedorczuk / Leonid A Sazanov / |
PubMed 要旨 | Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) ...Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIIICIV (the respirasome)-a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 Å and 6.7 Å, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII at 7.8 Å resolution. All observed density can be attributed to the known 80 subunits of the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain. |
リンク | Nature / PubMed:27654913 |
手法 | EM (単粒子) |
解像度 | 5.8 - 7.8 Å |
構造データ | EMDB-8128: Architecture of ovine respiratory supercomplex (I-III2-IV) conformation loose |
化合物 | ChemComp-SF4: ChemComp-FES: ChemComp-HEM: ChemComp-CU: ChemComp-HEA: ChemComp-CUA: |
由来 |
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キーワード | ELECTRON TRANSPORT / membrane protein complex / supercomplex / respirasome / MEMBRANE PROTEIN / Mitochondrial supercomplex / NADH-ubiquinone oxidoreductase / coenzyme Q-cytochrome c Oxidoreductase / cytochrome c oxidase |