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-Structure paper
タイトル | Structure of an RNA polymerase II preinitiation complex. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 112, Issue 44, Page 13543-13548, Year 2015 |
掲載日 | 2015年11月3日 |
著者 | Kenji Murakami / Kuang-Lei Tsai / Nir Kalisman / David A Bushnell / Francisco J Asturias / Roger D Kornberg / |
PubMed 要旨 | The structure of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM and image processing at a resolution of 6-11 Å. Atomic structures of over 50% of the ...The structure of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM and image processing at a resolution of 6-11 Å. Atomic structures of over 50% of the mass were fitted into the electron density map in a manner consistent with protein-protein cross-links previously identified by mass spectrometry. The resulting model of the PIC confirmed the main conclusions from previous cryo-EM at lower resolution, including the association of promoter DNA only with general transcription factors and not with the polymerase. Electron density due to DNA was identifiable by the grooves of the double helix and exhibited sharp bends at points downstream of the TATA box, with an important consequence: The DNA at the downstream end coincides with the DNA in a transcribing polymerase. The structure of the PIC is therefore conducive to promoter melting, start-site scanning, and the initiation of transcription. |
リンク | Proc Natl Acad Sci U S A / PubMed:26483468 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 6.0 - 11.0 Å |
構造データ | EMDB-3114: Structure of an RNA polymerase II pre-initiation complex EMDB-3115: |
化合物 | ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | TRANSCRIPTION / PRE-INITIATION COMPLEX / RNA POLYMERASE / TFIIE / TFIIH / TFIIB / TBP / TFIIF / PROTEIN |