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-Structure paper
タイトル | High-resolution structure determination by continuous-rotation data collection in MicroED. |
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ジャーナル・号・ページ | Nat Methods, Vol. 11, Issue 9, Page 927-930, Year 2014 |
掲載日 | 2014年8月3日 |
著者 | Brent L Nannenga / Dan Shi / Andrew G W Leslie / Tamir Gonen / |
PubMed 要旨 | MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous ...MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology. |
リンク | Nat Methods / PubMed:25086503 / PubMed Central |
手法 | EM (電子線結晶学) |
解像度 | 2.5 - 2.501 Å |
構造データ | |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | HYDROLASE / MICROED |