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-Structure paper
タイトル | Integrated molecular mechanism directing nucleosome reorganization by human FACT. |
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ジャーナル・号・ページ | Genes Dev, Vol. 30, Issue 6, Page 673-686, Year 2016 |
掲載日 | 2016年3月15日 |
著者 | Yasuo Tsunaka / Yoshie Fujiwara / Takuji Oyama / Susumu Hirose / Kosuke Morikawa / |
PubMed 要旨 | Facilitates chromatin transcription (FACT) plays essential roles in chromatin remodeling during DNA transcription, replication, and repair. Our structural and biochemical studies of human FACT- ...Facilitates chromatin transcription (FACT) plays essential roles in chromatin remodeling during DNA transcription, replication, and repair. Our structural and biochemical studies of human FACT-histone interactions present precise views of nucleosome reorganization, conducted by the FACT-SPT16 (suppressor of Ty 16) Mid domain and its adjacent acidic AID segment. AID accesses the H2B N-terminal basic region exposed by partial unwrapping of the nucleosomal DNA, thereby triggering the invasion of FACT into the nucleosome. The crystal structure of the Mid domain complexed with an H3-H4 tetramer exhibits two separate contact sites; the Mid domain forms a novel intermolecular β structure with H4. At the other site, the Mid-H2A steric collision on the H2A-docking surface of the H3-H4 tetramer within the nucleosome induces H2A-H2B displacement. This integrated mechanism results in disrupting the H3 αN helix, which is essential for retaining the nucleosomal DNA ends, and hence facilitates DNA stripping from histone. |
リンク | Genes Dev / PubMed:26966247 / PubMed Central |
手法 | X線回折 |
解像度 | 1.923 - 2.981 Å |
構造データ | PDB-4z2m: PDB-4z2n: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | Transcription/DNA binding protein / Transcription / Transcription-DNA binding protein complex |