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-Structure paper
タイトル | Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. |
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ジャーナル・号・ページ | Acta Crystallogr D Biol Crystallogr, Vol. 71, Issue Pt 4, Page 969-985, Year 2015 |
掲載日 | 2015年3月27日 |
![]() | Noelia Alonso-García / Inés García-Rubio / José A Manso / Rubén M Buey / Hector Urien / Arnoud Sonnenberg / Gunnar Jeschke / José M de Pereda / ![]() ![]() ![]() |
PubMed 要旨 | Integrin α6β4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin α6β4 has also been implicated in cell ...Integrin α6β4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin α6β4 has also been implicated in cell proliferation and migration and in carcinoma progression. The third and fourth fibronectin type III domains (FnIII-3,4) of integrin β4 mediate binding to the hemidesmosomal proteins BPAG1e and BPAG2, and participate in signalling. Here, it is demonstrated that X-ray crystallography, small-angle X-ray scattering and double electron-electron resonance (DEER) complement each other to solve the structure of the FnIII-3,4 region. The crystal structures of the individual FnIII-3 and FnIII-4 domains were solved and the relative arrangement of the FnIII domains was elucidated by combining DEER with site-directed spin labelling. Multiple structures of the interdomain linker were modelled by Monte Carlo methods complying with DEER constraints, and the final structures were selected against experimental scattering data. FnIII-3,4 has a compact and cambered flat structure with an evolutionary conserved surface that is likely to correspond to a protein-interaction site. Finally, this hybrid method is of general application for the study of other macromolecules and complexes. |
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手法 | SAS (X-ray synchrotron) / X線回折 |
解像度 | 1.5 - 1.606 Å |
構造データ | ![]() SASDAT6: ![]() PDB-4wtw: ![]() PDB-4wtx: |
化合物 | ![]() ChemComp-SO4: ![]() ChemComp-1PE: ![]() ChemComp-EDO: ![]() ChemComp-HOH: |
由来 |
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![]() | CELL ADHESION / IMMUNOGLOBULIN FOLD / FIBRONECTIN TYPE III / INTEGRIN / RECEPTOR |