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-Structure paper
タイトル | Study of the structural dynamics of the E coli 70S ribosome using real-space refinement. |
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ジャーナル・号・ページ | Cell, Vol. 113, Issue 6, Page 789-801, Year 2003 |
掲載日 | 2003年6月13日 |
![]() | Haixiao Gao / Jayati Sengupta / Mikel Valle / Andrei Korostelev / Narayanan Eswar / Scott M Stagg / Patrick Van Roey / Rajendra K Agrawal / Stephen C Harvey / Andrej Sali / Michael S Chapman / Joachim Frank / ![]() |
PubMed 要旨 | Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting ...Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation. |
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手法 | EM (単粒子) |
解像度 | 11.5 - 12.3 Å |
構造データ | ![]() PDB-4v47: ![]() PDB-4v48: |
由来 |
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![]() | RIBOSOME / EF-G.GTP-bound state / ratchet-like movement / real-space refinement |