EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
ジャーナル・号・ページ	J Biol Chem, Vol. 290, Issue 5, Page 2644-2658, Year 2015
掲載日	2015年1月30日
著者	Hubert Mayerhofer / Saravanan Panneerselvam / Heidi Kaljunen / Anne Tuukkanen / Haydyn D T Mertens / Jochen Mueller-Dieckmann /
PubMed 要旨	Ethylene initiates important aspects of plant growth and development through disulfide-linked receptor dimers located in the endoplasmic reticulum. The receptors feature a small transmembrane, ...Ethylene initiates important aspects of plant growth and development through disulfide-linked receptor dimers located in the endoplasmic reticulum. The receptors feature a small transmembrane, ethylene binding domain followed by a large cytosolic domain, which serves as a scaffold for the assembly of large molecular weight complexes of different ethylene receptors and other cellular participants of the ethylene signaling pathway. Here we report the crystallographic structures of the ethylene receptor 1 (ETR1) catalytic ATP-binding and the ethylene response sensor 1 dimerization histidine phosphotransfer (DHp) domains and the solution structure of the entire cytosolic domain of ETR1, all from Arabidopsis thaliana. The isolated dimeric ethylene response sensor 1 DHp domain is asymmetric, the result of different helical bending angles close to the conserved His residue. The structures of the catalytic ATP-binding, DHp, and receiver domains of ethylene receptors and of a homologous, but dissimilar, GAF domain were refined against experimental small angle x-ray scattering data, leading to a structural model of the entire cytosolic domain of the ethylene receptor 1. The model illustrates that the cytosolic domain is shaped like a dumbbell and that the receiver domain is flexible and assumes a position different from those observed in prokaryotic histidine kinases. Furthermore the cytosolic domain of ETR1 plays a key role, interacting with all other receptors and several participants of the ethylene signaling pathway. Our model, therefore, provides the first step toward a detailed understanding of the molecular mechanics of this important signal transduction process in plants.
リンク	J Biol Chem / PubMed:25451923 / PubMed Central
手法	SAS (X-ray synchrotron) / X線回折
解像度	1.9 - 2.15 Å
構造データ	SASDC42: Ethylene Receptor 1 Cytosolic Domain (Ethylene Receptor 1, ETR1(-ΔTM)) 手法: SAXS/SANS SASDCA7: Ethylene Receptor 1 (DHp + CA domains) (Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains, ETR1_DHp-CA) 手法: SAXS/SANS PDB-4mt8: Structure of the ERS1 dimerization and histidine phosphotransfer domain from Arabidopsis thaliana 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å PDB-4mtx: Structure of the ERS1 dimerization and histidine phosphotransfer domain from Arabidopsis thaliana 手法: X-RAY DIFFRACTION / 解像度: 2.15 Å PDB-4pl9: Structure of the catalytic domain of ETR1 from Arabidopsis thaliana 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å
化合物	ChemComp-HOH: WATER ChemComp-CD: Unknown entry ChemComp-CL: Unknown entry / クロリド ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ACT*: ACETATE ION / 酢酸イオン
由来	arabidopsis thaliana (シロイヌナズナ)
キーワード	TRANSFERASE / Four helix bundle / Histidine kinase / CTR1 / ER Membrane / ETR1 / ethylene receptor / Cadmium / ADP