EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement.
ジャーナル・号・ページ	Cell, Vol. 158, Issue 1, Page 121-131, Year 2014
掲載日	2014年7月3日
著者	Tatyana V Budkevich / Jan Giesebrecht / Elmar Behrmann / Justus Loerke / David J F Ramrath / Thorsten Mielke / Jochen Ismer / Peter W Hildebrand / Chang-Shung Tung / Knud H Nierhaus / Karissa Y Sanbonmatsu / Christian M T Spahn /
PubMed 要旨	The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution ...The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1A⋅Val-tRNA⋅GMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection.
リンク	Cell / PubMed:24995983 / PubMed Central
手法	EM (単粒子)
解像度	6.9 - 8.9 Å
構造データ	2620 4uje EMDB-2620: POST-translocational 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement PDB-4uje: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-2621: PRE-translocational (classical-1) 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-2622: PRE-translocational (classical-2) 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement 手法: EM (単粒子) / 解像度: 7.5 Å 2623 4cxg EMDB-2623: Sampling intermediate of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement PDB-4cxg: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement 手法: EM (単粒子) / 解像度: 8.7 Å 2624 4cxh EMDB-2624: Recognition intermediate of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement PDB-4cxh: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement 手法: EM (単粒子) / 解像度: 8.9 Å
化合物	ChemComp-PHE: PHENYLALANINE / フェニルアラニン
由来	oryctolagus cuniculus (ウサギ) escherichia coli (大腸菌) bos taurus (ウシ)
キーワード	TRANSLATION / MAMMALIAN 80S RIBOSOME / ELONGATION CYCLE / TRNA SELECTION / EUKARYOTIC TERNARY COMPLEX / ELONGATION FACTOR EEF1A / RIBOSOME / POST-TRANSLOCATIONAL STATE