EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Tracking in atomic detail the functional specializations in viral RecA helicases that occur during evolution.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 41, Issue 20, Page 9396-9410, Year 2013
掲載日	2013年8月11日
著者	Kamel El Omari / Christoph Meier / Denis Kainov / Geoff Sutton / Jonathan M Grimes / Minna M Poranen / Dennis H Bamford / Roman Tuma / David I Stuart / Erika J Mancini /
PubMed 要旨	Many complex viruses package their genomes into empty protein shells and bacteriophages of the Cystoviridae family provide some of the simplest models for this. The cystoviral hexameric NTPase, P4, ...Many complex viruses package their genomes into empty protein shells and bacteriophages of the Cystoviridae family provide some of the simplest models for this. The cystoviral hexameric NTPase, P4, uses chemical energy to translocate single-stranded RNA genomic precursors into the procapsid. We previously dissected the mechanism of RNA translocation for one such phage, 12, and have now investigated three further highly divergent, cystoviral P4 NTPases (from 6, 8 and 13). High-resolution crystal structures of the set of P4s allow a structure-based phylogenetic analysis, which reveals that these proteins form a distinct subfamily of the RecA-type ATPases. Although the proteins share a common catalytic core, they have different specificities and control mechanisms, which we map onto divergent N- and C-terminal domains. Thus, the RNA loading and tight coupling of NTPase activity with RNA translocation in 8 P4 is due to a remarkable C-terminal structure, which wraps right around the outside of the molecule to insert into the central hole where RNA binds to coupled L1 and L2 loops, whereas in 12 P4, a C-terminal residue, serine 282, forms a specific hydrogen bond to the N7 of purines ring to confer purine specificity for the 12 enzyme.
リンク	Nucleic Acids Res / PubMed:23939620 / PubMed Central
手法	X線回折
解像度	1.7 - 3.1 Å
構造データ	PDB-4blo: P4 PROTEIN FROM BACTERIOPHAGE PHI6 IN COMPLEX WITH ADP 手法: X-RAY DIFFRACTION / 解像度: 2.8 Å PDB-4blp: P4 PROTEIN FROM BACTERIOPHAGE PHI13 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å PDB-4blq: P4 PROTEIN FROM BACTERIOPHAGE PHI8 手法: X-RAY DIFFRACTION / 解像度: 2.79 Å PDB-4blr: P4 PROTEIN FROM BACTERIOPHAGE PHI12 IN COMPLEX WITH UTP 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å PDB-4bls: P4 PROTEIN FROM BACTERIOPHAGE PHI12 Q278A MUTANT IN COMPLEX WITH AMPcPP 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-4blt: P4 PROTEIN FROM BACTERIOPHAGE PHI12 S292A MUTANT IN COMPLEX WITH AMPcPP 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-4bwy: P4 PROTEIN FROM BACTERIOPHAGE PHI8 (R32) 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-FLC: CITRATE ANION / シトラ-ト ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-HOH: WATER ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP / UTPYM ChemComp-DTT: 2,3-DIHYDROXY-1,4-DITHIOBUTANE / L-DTT ChemComp-APC: DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / α,β-メチレンATP / AMP-CPP, エネルギー貯蔵分子類似体*YM
由来	pseudomonas phage phi6 (ファージ) pseudomonas phage phi13 (ファージ) pseudomonas phage phi8 (ファージ) pseudomonas phage phi12 (ファージ)
キーワード	HYDROLASE / ATPASE / CYSTOVIRIDAE / NTPASE / PACKAGING