EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A putative ATPase mediates RNA transcription and capping in a dsRNA virus.
ジャーナル・号・ページ	Elife, Vol. 4, Page e07901, Year 2015
掲載日	2015年8月4日
著者	Xuekui Yu / Jiansen Jiang / Jingchen Sun / Z Hong Zhou /
PubMed 要旨	mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high- ...mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.9-3.1 Å) cryo-electron microscopy structures of cytoplasmic polyhedrosis virus with bound ligands, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein (TP) also has an ATP-binding site and is likely an ATPase. S-adenosyl-L-methionine (SAM) acts as a signal and binds the methylase-2 domain of TP to induce conformational change of the viral capsid, which in turn activates the putative ATPase. ATP binding/hydrolysis leads to an enlarged capsid for efficient mRNA synthesis, an open GTase domain for His217-mediated guanylyl transfer, and an open methylase-1 domain for SAM binding and methyl transfer. Taken together, our data support a role of the putative ATPase in mediating the activation of mRNA transcription and capping within the confines of the virus.
リンク	Elife / PubMed:26240998 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.1 Å
構造データ	6371 3jaz EMDB-6371: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jaz: Atomic model of cytoplasmic polyhedrosis virus with ATP 手法: EM (単粒子) / 解像度: 3.1 Å 6374 3jb0 EMDB-6374: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jb0: Atomic model of cytoplasmic polyhedrosis virus with GTP 手法: EM (単粒子) / 解像度: 2.9 Å 6375 3jb1 EMDB-6375: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jb1: Atomic model of cytoplasmic polyhedrosis virus with SAM 手法: EM (単粒子) / 解像度: 3.1 Å 6376 3jb2 EMDB-6376: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jb2: Atomic model of cytoplasmic polyhedrosis virus with SAM and GTP 手法: EM (単粒子) / 解像度: 3.1 Å 6377 3jb3 EMDB-6377: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jb3: Atomic model of cytoplasmic polyhedrosis virus with SAM, GTP and ATP 手法: EM (単粒子) / 解像度: 3.1 Å 6378 3jay EMDB-6378: A putative ATPase mediates RNA transcription and capping in a dsRNA virus PDB-3jay: Atomic model of transcribing cytoplasmic polyhedrosis virus 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	bombyx mori cypovirus 1 (ウイルス)
キーワード	VIRUS / viral ATPase / histidine-mediated guanylyl transfer / conformational changes / regulation of transcription