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-Structure paper
タイトル | Remodeling of actin filaments by ADF/cofilin proteins. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 108, Issue 51, Page 20568-20572, Year 2011 |
掲載日 | 2011年12月20日 |
著者 | Vitold E Galkin / Albina Orlova / Dmitri S Kudryashov / Alexander Solodukhin / Emil Reisler / Gunnar F Schröder / Edward H Egelman / |
PubMed 要旨 | Cofilin/ADF proteins play key roles in the dynamics of actin, one of the most abundant and highly conserved eukaryotic proteins. We used cryoelectron microscopy to generate a 9-Å resolution three- ...Cofilin/ADF proteins play key roles in the dynamics of actin, one of the most abundant and highly conserved eukaryotic proteins. We used cryoelectron microscopy to generate a 9-Å resolution three-dimensional reconstruction of cofilin-decorated actin filaments, the highest resolution achieved for a complex of F-actin with an actin-binding protein. We show that the cofilin-induced change in the filament twist is due to a unique conformation of the actin molecule unrelated to any previously observed state. The changes between the actin protomer in naked F-actin and in the actin-cofilin filament are greater than the conformational changes between G- and F-actin. Our results show the structural plasticity of actin, suggest that other actin-binding proteins may also induce large but different conformational changes, and show that F-actin cannot be described by a single molecular model. |
リンク | Proc Natl Acad Sci U S A / PubMed:22158895 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 9.0 Å |
構造データ | |
由来 |
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キーワード | CONTRACTILE PROTEIN/PROTEIN BINDING / helical polymer / CONTRACTILE PROTEIN-ACTIN BINDING PROTEIN complex / CONTRACTILE PROTEIN-PROTEIN BINDING complex |