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-Structure paper
タイトル | Structure of a bacterial type IV secretion core complex at subnanometre resolution. |
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ジャーナル・号・ページ | EMBO J, Vol. 32, Issue 8, Page 1195-1204, Year 2013 |
掲載日 | 2013年4月17日 |
著者 | Angel Rivera-Calzada / Rémi Fronzes / Christos G Savva / Vidya Chandran / Pei W Lian / Toon Laeremans / Els Pardon / Jan Steyaert / Han Remaut / Gabriel Waksman / Elena V Orlova / |
PubMed 要旨 | Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and ...Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived. |
リンク | EMBO J / PubMed:23511972 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 8.5 - 12.4 Å |
構造データ | EMDB-2232: Full-length structure of the bacterial pKM101 type IV secretion system core complex EMDB-2233: Subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase |
由来 |
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キーワード | MEMBRANE PROTEIN / BACTERIAL SECRETION / TYPE IV SECRETION / CELL ADHESION |