EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.
ジャーナル・号・ページ	J Struct Biol, Vol. 138, Issue 1-2, Page 92-9104, Year 2002
掲載日	2003年7月18日
著者	Li Fan Chen / Hanspeter Winkler / Michael K Reedy / Mary C Reedy / Kenneth A Taylor /
PubMed 要旨	Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), ...Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.
リンク	J Struct Biol / PubMed:12160705
手法	EM (トモグラフィー)
解像度	70 Å
構造データ	PDB-1m8q: Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1mvw: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o18: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o19: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1a: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1b: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1c: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1d: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1e: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1f: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å PDB-1o1g: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE 手法: ELECTRON MICROSCOPY / 解像度: 70 Å
由来	gallus gallus (ニワトリ) oryctolagus cuniculus (ウサギ)
キーワード	CONTRACTILE PROTEIN / Actin-myosin complex in situ in muscle