EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dynamic rotation of the protruding domain enhances the infectivity of norovirus.
ジャーナル・号・ページ	PLoS Pathog, Vol. 16, Issue 7, Page e1008619, Year 2020
掲載日	2020年7月2日
著者	Chihong Song / Reiko Takai-Todaka / Motohiro Miki / Kei Haga / Akira Fujimoto / Ryoka Ishiyama / Kazuki Oikawa / Masaru Yokoyama / Naoyuki Miyazaki / Kenji Iwasaki / Kosuke Murakami / Kazuhiko Katayama / Kazuyoshi Murata /
PubMed 要旨	Norovirus is the major cause of epidemic nonbacterial gastroenteritis worldwide. Lack of structural information on infection and replication mechanisms hampers the development of effective vaccines ...Norovirus is the major cause of epidemic nonbacterial gastroenteritis worldwide. Lack of structural information on infection and replication mechanisms hampers the development of effective vaccines and remedies. Here, using cryo-electron microscopy, we show that the capsid structure of murine noroviruses changes in response to aqueous conditions. By twisting the flexible hinge connecting two domains, the protruding (P) domain reversibly rises off the shell (S) domain in solutions of higher pH, but rests on the S domain in solutions of lower pH. Metal ions help to stabilize the resting conformation in this process. Furthermore, in the resting conformation, the cellular receptor CD300lf is readily accessible, and thus infection efficiency is significantly enhanced. Two similar P domain conformations were also found simultaneously in the human norovirus GII.3 capsid, although the mechanism of the conformational change is not yet clear. These results provide new insights into the mechanisms of non-enveloped norovirus transmission that invades host cells, replicates, and sometimes escapes the hosts immune system, through dramatic environmental changes in the gastrointestinal tract.
リンク	PLoS Pathog / PubMed:32614892 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 13.0 Å
構造データ	EMDB-9735: Cryo-EM structure of Murine Norovirus S7 virion with the resting P-domain conformation 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-9736: Cryo-EM structure of Murine Norovirus S7 virion with the rising P-domain conformation 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-9737: Cryo-EM structure of Murine Norovirus 1 virion with the resting P-domain conformation 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-9738: Cryo-EM structure of Murine Norovirus 1 virion with the rising P-domain conformation 手法: EM (単粒子) / 解像度: 7.3 Å EMDB-9739: Cryo-EM structure of Human Norovirus GII.3 VLP with the resting P-domain conformation 手法: EM (単粒子) / 解像度: 9.3 Å EMDB-9740: Cryo-EM structure of Human Norovirus GII.3 VLP with the rising P-domain conformation 手法: EM (単粒子) / 解像度: 13.0 Å 9741 6iuk EMDB-9741: Cryo-EM structure of Murine Norovirus S7 VLP PDB-6iuk: Cryo-EM structure of Murine Norovirus capsid 手法: EM (単粒子) / 解像度: 3.5 Å
由来	murine norovirus gv/nih-2410/2005/usa (ウイルス)
キーワード	VIRUS / VLP / mature / stable