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-Structure paper
タイトル | Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d. |
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ジャーナル・号・ページ | Cell, Vol. 175, Issue 1, Page 212-223.e17, Year 2018 |
掲載日 | 2018年9月20日 |
著者 | Cheng Zhang / Silvana Konermann / Nicholas J Brideau / Peter Lotfy / Xuebing Wu / Scott J Novick / Timothy Strutzenberg / Patrick R Griffin / Patrick D Hsu / Dmitry Lyumkis / |
PubMed 要旨 | CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems ...CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy structures of Cas13d-guide RNA binary complex and Cas13d-guide-target RNA ternary complex to 3.4 and 3.3 Å resolution, respectively. Furthermore, a 6.5 Å reconstruction of apo Cas13d combined with hydrogen-deuterium exchange revealed conformational dynamics that have implications for RNA scanning. These structures, together with biochemical and cellular characterization, provide insights into its RNA-guided, RNA-targeting mechanism and delineate a blueprint for the rational design of improved transcriptome engineering technologies. |
リンク | Cell / PubMed:30241607 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.3 - 6.5 Å |
構造データ | EMDB-9015: |
化合物 | ChemComp-MG: |
由来 |
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キーワード | RNA BINDING PROTEIN/RNA / CRISPR-Cas / RNase / Complex / RNA BINDING PROTEIN-RNA complex |