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-Structure paper
タイトル | Human β-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability. |
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ジャーナル・号・ページ | Dev Cell, Vol. 47, Issue 2, Page 175-190.e5, Year 2018 |
掲載日 | 2018年10月22日 |
著者 | Shih-Chieh Ti / Gregory M Alushin / Tarun M Kapoor / |
PubMed 要旨 | Cell biological studies have shown that protofilament number, a fundamental feature of microtubules, can correlate with the expression of different tubulin isotypes. However, it is not known if ...Cell biological studies have shown that protofilament number, a fundamental feature of microtubules, can correlate with the expression of different tubulin isotypes. However, it is not known if tubulin isotypes directly control this basic microtubule property. Here, we report high-resolution cryo-EM reconstructions (3.5-3.65 Å) of purified human α1B/β3 and α1B/β2B microtubules and find that the β-tubulin isotype can determine protofilament number. Comparisons of atomic models of 13- and 14-protofilament microtubules reveal how tubulin subunit plasticity, manifested in "accordion-like" distributed structural changes, can accommodate distinct lattice organizations. Furthermore, compared to α1B/β3 microtubules, α1B/β2B filaments are more stable to passive disassembly and against depolymerization by MCAK or chTOG, microtubule-associated proteins with distinct mechanisms of action. Mixing tubulin isotypes in different proportions results in microtubules with protofilament numbers and stabilities intermediate to those of isotypically pure filaments. Together, our findings indicate that microtubule protofilament number and stability can be controlled through β-tubulin isotype composition. |
リンク | Dev Cell / PubMed:30245156 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 3.5 - 3.65 Å |
構造データ | |
化合物 | ChemComp-MG: ChemComp-G2P: ChemComp-GTP: |
由来 |
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キーワード | STRUCTURAL PROTEIN / cytoskeleton / microtubules / recombinant human tubulin / tubulin isotype |