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-Structure paper
タイトル | Zika virus induced cellular remodelling. |
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ジャーナル・号・ページ | Cell Microbiol, Vol. 19, Issue 8, Year 2017 |
掲載日 | 2017年4月18日 |
著者 | Evan D Rossignol / Kristen N Peters / John H Connor / Esther Bullitt / |
PubMed 要旨 | Zika virus (ZIKV) has been associated with morbidities such as Guillain-Barré, infant microcephaly, and ocular disease. The spread of this positive-sense, single-stranded RNA virus and its growing ...Zika virus (ZIKV) has been associated with morbidities such as Guillain-Barré, infant microcephaly, and ocular disease. The spread of this positive-sense, single-stranded RNA virus and its growing public health threat underscore gaps in our understanding of basic ZIKV virology. To advance knowledge of the virus replication cycle within mammalian cells, we use serial section 3-dimensional electron tomography to demonstrate the widespread remodelling of intracellular membranes upon infection with ZIKV. We report extensive structural rearrangements of the endoplasmic reticulum and reveal stages of the ZIKV viral replication cycle. Structures associated with RNA genome replication and virus assembly are observed integrated within the endoplasmic reticulum, and we show viruses in transit through the Golgi apparatus for viral maturation, and subsequent cellular egress. This study characterises in detail the 3-dimensional ultrastructural organisation of the ZIKV replication cycle stages. Our results show close adherence of the ZIKV replication cycle to the existing flavivirus replication paradigm. |
リンク | Cell Microbiol / PubMed:28318141 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-8654: EMDB-8655: EMDB-8656: EMDB-8657: |
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