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-Structure paper
タイトル | Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 2, Page 16269, Year 2017 |
掲載日 | 2017年2月6日 |
著者 | Yi-Wei Chang / Andreas Kjær / Davi R Ortega / Gabriela Kovacikova / John A Sutherland / Lee A Rettberg / Ronald K Taylor / Grant J Jensen / |
PubMed 要旨 | Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal ...Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal body. Based on pilin features, T4P are classified into type IVa pili (T4aP) and type IVb pili (T4bP). T4aP are more widespread and are involved in cell motility, DNA transfer, host predation and electron transfer. T4bP are less prevalent and are mainly found in enteropathogenic bacteria, where they play key roles in host colonization. Following similar work on T4aP machines, here we use electron cryotomography to reveal the three-dimensional in situ structure of a T4bP machine in its piliated and non-piliated states. The specific machine we analyse is the Vibrio cholerae toxin-coregulated pilus machine (TCPM). Although only about half of the components of the TCPM show sequence homology to components of the previously analysed Myxococcus xanthus T4aP machine (T4aPM), we find that their structures are nevertheless remarkably similar. Based on homologies with components of the M. xanthus T4aPM and additional reconstructions of TCPM mutants in which the non-homologous proteins are individually deleted, we propose locations for all eight TCPM components within the complex. Non-homologous proteins in the T4aPM and TCPM are found to form similar structures, suggesting new hypotheses for their functions and evolutionary histories. |
リンク | Nat Microbiol / PubMed:28165453 / PubMed Central |
手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
解像度 | 50.0 Å |
構造データ | EMDB-3792: EMDB-8492: EMDB-8493: EMDB-8494: EMDB-8495: EMDB-8496: EMDB-8497: EMDB-8498: EMDB-8499: EMDB-8500: EMDB-8501: EMDB-8502: EMDB-8503: |
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