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-Structure paper
タイトル | Genetic, Structural, and Phenotypic Properties of MS2 Coliphage with Resistance to ClO Disinfection. |
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ジャーナル・号・ページ | Environ Sci Technol, Vol. 50, Issue 24, Page 13520-13528, Year 2016 |
掲載日 | 2016年12月20日 |
![]() | Qingxia Zhong / Anna Carratalà / Sergey Nazarov / Ricardo Cesar Guerrero-Ferreira / Laura Piccinini / Virginie Bachmann / Petr G Leiman / Tamar Kohn / ![]() |
PubMed 要旨 | Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has been devoted to characterizing disinfection resistance. Here, we investigated the ...Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has been devoted to characterizing disinfection resistance. Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine dioxide (ClO). ClO inactivates MS2 by degrading its structural proteins, thereby disrupting the ability of MS2 to attach to and infect its host. ClO-resistant virus populations emerged not only after repeated cycles of ClO disinfection followed by regrowth but also after dilution-regrowth cycles in the absence of ClO. The resistant populations exhibited several fixed mutations which caused the substitution of ClO-labile by ClO-stable amino acids. On a phenotypic level, these mutations resulted in a more stable host binding during inactivation compared to the wild-type, thus resulting in a greater ability to maintain infectivity. This conclusion was supported by cryo-electron microscopy reconstruction of the virus particle, which demonstrated that most structural modification occurred in the putative A protein, an important binding factor. Resistance was specific to the inactivation mechanism of ClO and did not result in significant cross-resistance to genome-damaging disinfectants. Overall, our data indicate that resistant viruses may emerge even in the absence of ClO pressure but that they can be inactivated by other common disinfectants. |
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手法 | EM (単粒子) |
解像度 | 9.5 - 10.5 Å |
構造データ | ![]() EMDB-8360: ![]() EMDB-8396: |