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-Structure paper
タイトル | Structural Heterogeneity in Pre-40S Ribosomes. |
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ジャーナル・号・ページ | Structure, Vol. 25, Issue 2, Page 329-340, Year 2017 |
掲載日 | 2017年2月7日 |
著者 | Matthew C Johnson / Homa Ghalei / Katelyn A Doxtader / Katrin Karbstein / M Elizabeth Stroupe / |
PubMed 要旨 | Late-stage 40S ribosome assembly is a highly regulated dynamic process that occurs in the cytoplasm, alongside the full translation machinery. Seven assembly factors (AFs) regulate and facilitate ...Late-stage 40S ribosome assembly is a highly regulated dynamic process that occurs in the cytoplasm, alongside the full translation machinery. Seven assembly factors (AFs) regulate and facilitate maturation, but the mechanisms through which they work remain undetermined. Here, we present a series of structures of the immature small subunit (pre-40S) determined by three-dimensional (3D) cryoelectron microscopy with 3D sorting to assess the molecule's heterogeneity. These structures demonstrate an extensive structural heterogeneity of interface AFs that likely regulates subunit joining during 40S maturation. We also present structural models for the beak and the platform, two regions where the low resolution of previous studies did not allow for localization of AFs and the rRNA, respectively. These models are supported by biochemical analyses using point variants and suggest that maturation of the 18S 3' end is regulated by dissociation of the AF Dim1 from the subunit interface, consistent with previous biochemical analyses. |
リンク | Structure / PubMed:28111018 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 9.4 - 10.4 Å |
構造データ | EMDB-8346: EMDB-8347: EMDB-8348: EMDB-8349: EMDB-8350: EMDB-8351: EMDB-8352: EMDB-8353: |
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