EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1.
ジャーナル・号・ページ	Nat Med, Vol. 24, Issue 6, Page 857-867, Year 2018
掲載日	2018年6月4日
著者	Kai Xu / Priyamvada Acharya / Rui Kong / Cheng Cheng / Gwo-Yu Chuang / Kevin Liu / Mark K Louder / Sijy O'Dell / Reda Rawi / Mallika Sastry / Chen-Hsiang Shen / Baoshan Zhang / Tongqing Zhou / Mangaiarkarasi Asokan / Robert T Bailer / Michael Chambers / Xuejun Chen / Chang W Choi / Venkata P Dandey / Nicole A Doria-Rose / Aliaksandr Druz / Edward T Eng / S Katie Farney / Kathryn E Foulds / Hui Geng / Ivelin S Georgiev / Jason Gorman / Kurt R Hill / Alexander J Jafari / Young D Kwon / Yen-Ting Lai / Thomas Lemmin / Krisha McKee / Tiffany Y Ohr / Li Ou / Dongjun Peng / Ariana P Rowshan / Zizhang Sheng / John-Paul Todd / Yaroslav Tsybovsky / Elise G Viox / Yiran Wang / Hui Wei / Yongping Yang / Amy F Zhou / Rui Chen / Lu Yang / Diana G Scorpio / Adrian B McDermott / Lawrence Shapiro / Bridget Carragher / Clinton S Potter / John R Mascola / Peter D Kwong /
PubMed 要旨	A central goal of HIV-1 vaccine research is the elicitation of antibodies capable of neutralizing diverse primary isolates of HIV-1. Here we show that focusing the immune response to exposed N- ...A central goal of HIV-1 vaccine research is the elicitation of antibodies capable of neutralizing diverse primary isolates of HIV-1. Here we show that focusing the immune response to exposed N-terminal residues of the fusion peptide, a critical component of the viral entry machinery and the epitope of antibodies elicited by HIV-1 infection, through immunization with fusion peptide-coupled carriers and prefusion stabilized envelope trimers, induces cross-clade neutralizing responses. In mice, these immunogens elicited monoclonal antibodies capable of neutralizing up to 31% of a cross-clade panel of 208 HIV-1 strains. Crystal and cryoelectron microscopy structures of these antibodies revealed fusion peptide conformational diversity as a molecular explanation for the cross-clade neutralization. Immunization of guinea pigs and rhesus macaques induced similarly broad fusion peptide-directed neutralizing responses, suggesting translatability. The N terminus of the HIV-1 fusion peptide is thus a promising target of vaccine efforts aimed at eliciting broadly neutralizing antibodies.
リンク	Nat Med / PubMed:29867235 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.55 - 19.6 Å
構造データ	7459 6cde EMDB-7459, PDB-6cde: Cryo-EM structure at 3.8 A resolution of vaccine-elicited antibody vFP20.01 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.8 Å 7460 6cdi EMDB-7460, PDB-6cdi: Cryo-EM structure at 3.6 A resolution of vaccine-elicited antibody vFP16.02 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-8420: Cryo-EM structure of BG505 DS-SOSIP HIV-1 Env trimer in complex with vaccine elicited, fusion peptide-directed antibody vFP1.01 手法: EM (単粒子) / 解像度: 8.58 Å EMDB-8421: Cryo-EM structure of an asymmetric complex of BG505 DS-SOSIP HIV-1 Env trimer with vaccine elicited, fusion peptide-directed antibody vFP5.01 手法: EM (単粒子) / 解像度: 14.7 Å EMDB-8422: Cryo-EM structure of an asymmetric complex of BG505 DS-SOSIP HIV-1 Env trimer with vaccine elicited, fusion peptide-directed antibody vFP5.01 手法: EM (単粒子) / 解像度: 19.6 Å PDB-5tkj: Structure of vaccine-elicited diverse HIV-1 neutralizing antibody vFP1.01 in complex with HIV-1 fusion peptide residue 512-519 手法: X-RAY DIFFRACTION / 解像度: 2.118 Å PDB-5tkk: Structure of mouse vaccination-elicited HIV neutralizing antibody vFP5.01 in complex with HIV-1 fusion peptide residue 512-519 手法: X-RAY DIFFRACTION / 解像度: 1.55 Å PDB-6cdm: Structure of vaccine-elicited HIV-1 neutralizing antibody vFP7.04 in complex with HIV-1 fusion peptide residue 512-519 手法: X-RAY DIFFRACTION / 解像度: 2.408 Å PDB-6cdo: Structure of vaccine-elicited HIV-1 neutralizing antibody vFP16.02 in complex with HIV-1 fusion peptide residue 512-519 手法: X-RAY DIFFRACTION / 解像度: 2.099 Å PDB-6cdp: Vaccine-elicited HIV-1 neutralizing antibody vFP20.01 in complex with HIV-1 fusion peptide residue 512-519 手法: X-RAY DIFFRACTION / 解像度: 2.456 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス
由来	homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス) mus musculus (ハツカネズミ)
キーワード	IMMUNE SYSTEM / Neutralizing / antibody / fusion-peptide / complex / mouse / VIRAL PROTEIN / HIV-1 Env / BG505 SOSIP / fusion peptide / VRC03 / PGT122 / vFP20.01 / vFP16.02