EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 25, Issue 2, Page 154-162, Year 2018
掲載日	2018年1月29日
著者	Simon Poepsel / Vignesh Kasinath / Eva Nogales /
PubMed 要旨	Epigenetic regulation is mediated by protein complexes that couple recognition of chromatin marks to activity or recruitment of chromatin-modifying enzymes. Polycomb repressive complex 2 (PRC2), a ...Epigenetic regulation is mediated by protein complexes that couple recognition of chromatin marks to activity or recruitment of chromatin-modifying enzymes. Polycomb repressive complex 2 (PRC2), a gene silencer that methylates lysine 27 of histone H3, is stimulated upon recognition of its own catalytic product and has been shown to be more active on dinucleosomes than H3 tails or single nucleosomes. These properties probably facilitate local H3K27me2/3 spreading, causing heterochromatin formation and gene repression. Here, cryo-EM reconstructions of human PRC2 bound to bifunctional dinucleosomes show how a single PRC2, via interactions with nucleosomal DNA, positions the H3 tails of the activating and substrate nucleosome to interact with the EED subunit and the SET domain of EZH2, respectively. We show how the geometry of the PRC2-DNA interactions allows PRC2 to accommodate varying lengths of the linker DNA between nucleosomes. Our structures illustrate how an epigenetic regulator engages with a complex chromatin substrate.
リンク	Nat Struct Mol Biol / PubMed:29379173 / PubMed Central
手法	EM (単粒子)
解像度	4.6 - 13.3 Å
構造データ	EMDB-7306: Structure of PRC2 bound to a H3K27me3/WT hetero-dinucleosome substrate with a 35 bp DNA linker 手法: EM (単粒子) / 解像度: 6.2 Å EMDB-7307: Structure of PRC2 bound to a H3K27me3/WT hetero-dinucleosome substrate with a 30 bp DNA linker 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-7308: Structure of PRC2 bound to a H3K27me3/WT hetero-dinucleosome substrate with a 40 bp DNA linker 手法: EM (単粒子) / 解像度: 13.3 Å EMDB-7309: Structure of human PRC2 bound to a hetero-dinucleosome substrate with 35 bp linker DNA. 3D classification of signal subtracted particles lacking the unmodified substrate nucleosome. Example Class1. 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-7310: Structure of human PRC2 bound to a hetero-dinucleosome substrate with 35 bp linker DNA. 3D classification of signal subtracted particles lacking the unmodified substrate nucleosome. Example Class3. 手法: EM (単粒子) / 解像度: 10.1 Å EMDB-7311: Structure of PRC2 bound to a H3K27me3/WT hetero-dinucleosome substrate with a 35 bp DNA linker. Masked refinement of PRC2-substrate nucleosome subcomplex. 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-7312: Structure of human PRC2 bound to a hetero-dinucleosome substrate with 35 bp linker DNA. Refinement after signal subtraction of the modified nucleosome ton improve resolution of the substrate nucleosome - PRC2 interface. 手法: EM (単粒子) / 解像度: 7.7 Å EMDB-7313: Structure of human PRC2-AEBP2 手法: EM (単粒子) / 解像度: 4.6 Å
由来	Homo sapiens (ヒト)