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-Structure paper
タイトル | Structural delineation of a quaternary, cleavage-dependent epitope at the gp41-gp120 interface on intact HIV-1 Env trimers. |
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ジャーナル・号・ページ | Immunity, Vol. 40, Issue 5, Page 669-680, Year 2014 |
掲載日 | 2014年5月15日 |
著者 | Claudia Blattner / Jeong Hyun Lee / Kwinten Sliepen / Ronald Derking / Emilia Falkowska / Alba Torrents de la Peña / Albert Cupo / Jean-Philippe Julien / Marit van Gils / Peter S Lee / Wenjie Peng / James C Paulson / Pascal Poignard / Dennis R Burton / John P Moore / Rogier W Sanders / Ian A Wilson / Andrew B Ward / |
PubMed 要旨 | All previously characterized broadly neutralizing antibodies to the HIV-1 envelope glycoprotein (Env) target one of four major sites of vulnerability. Here, we define and structurally characterize a ...All previously characterized broadly neutralizing antibodies to the HIV-1 envelope glycoprotein (Env) target one of four major sites of vulnerability. Here, we define and structurally characterize a unique epitope on Env that is recognized by a recently discovered family of human monoclonal antibodies (PGT151-PGT158). The PGT151 epitope is comprised of residues and glycans at the interface of gp41 and gp120 within a single protomer and glycans from both subunits of a second protomer and represents a neutralizing epitope that is dependent on both gp120 and gp41. Because PGT151 binds only to properly formed, cleaved trimers, this distinctive property, and its ability to stabilize Env trimers, has enabled the successful purification of mature, cleaved Env trimers from the cell surface as a complex with PGT151. Here we compare the structural and functional properties of membrane-extracted Env trimers from several clades with those of the soluble, cleaved SOSIP gp140 trimer. |
リンク | Immunity / PubMed:24768348 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.827 - 25.0 Å |
構造データ | EMDB-5918: EMDB-5919: EMDB-5920: EMDB-5921: PDB-4nug: PDB-4nuj: |
化合物 | ChemComp-P6G: ChemComp-HOH: |
由来 |
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キーワード | IMMUNE SYSTEM / immunoglobulin / Fab fragment / HIV Envelope |