EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.
ジャーナル・号・ページ	Protein Cell, Vol. 5, Issue 5, Page 394-407, Year 2014
掲載日	2014年3月28日
著者	Zhixiu Yang / Qiang Guo / Simon Goto / Yuling Chen / Ningning Li / Kaige Yan / Yixiao Zhang / Akira Muto / Haiteng Deng / Hyouta Himeno / Jianlin Lei / Ning Gao /
PubMed 要旨	The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA ...The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coli strain (∆rsgA∆rbfA). The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, intermediates derived under the two contrasting salt conditions (high and low) likely reflect two distinctive assembly stages, the relatively early and late stages of the 3' domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5' end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal the location of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mechanisms on subunit production and protein translation.
リンク	Protein Cell / PubMed:24671761 / PubMed Central
手法	EM (単粒子)
解像度	13.5 - 21.2 Å
構造データ	EMDB-5900: Cryo-EM structure of low salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-5904: Cryo-EM structure of low salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 13.5 Å EMDB-5905: Cryo-EM structure of low salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 14.4 Å EMDB-5906: Cryo-EM structure of low salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 17.8 Å EMDB-5907: Cryo-EM structure of low salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 14.3 Å EMDB-5908: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 21.2 Å EMDB-5909: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 15.2 Å EMDB-5910: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain 手法: EM (単粒子) / 解像度: 18.3 Å
由来	Escherichia coli (大腸菌)