EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.
ジャーナル・号・ページ	Science, Vol. 343, Issue 6176, Page 1247997, Year 2014
掲載日	2014年3月14日
著者	Martin Jinek / Fuguo Jiang / David W Taylor / Samuel H Sternberg / Emine Kaya / Enbo Ma / Carolin Anders / Michael Hauer / Kaihong Zhou / Steven Lin / Matias Kaplan / Anthony T Iavarone / Emmanuelle Charpentier / Eva Nogales / Jennifer A Doudna /
PubMed 要旨	Type II CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems use an RNA-guided DNA endonuclease, Cas9, to generate double-strand breaks in invasive DNA ...Type II CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems use an RNA-guided DNA endonuclease, Cas9, to generate double-strand breaks in invasive DNA during an adaptive bacterial immune response. Cas9 has been harnessed as a powerful tool for genome editing and gene regulation in many eukaryotic organisms. We report 2.6 and 2.2 angstrom resolution crystal structures of two major Cas9 enzyme subtypes, revealing the structural core shared by all Cas9 family members. The architectures of Cas9 enzymes define nucleic acid binding clefts, and single-particle electron microscopy reconstructions show that the two structural lobes harboring these clefts undergo guide RNA-induced reorientation to form a central channel where DNA substrates are bound. The observation that extensive structural rearrangements occur before target DNA duplex binding implicates guide RNA loading as a key step in Cas9 activation.
リンク	Science / PubMed:24505130 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.201 - 21.0 Å
構造データ	EMDB-5858: Structures of Cas9 endonucleases reveal RNA-mediated conformational activation 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-5859: Structures of Cas9 endonucleases reveal RNA-mediated conformational activation 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-5860: Structures of Cas9 endonucleases reveal RNA-mediated conformational activation 手法: EM (単粒子) / 解像度: 21.0 Å PDB-4cmp: Crystal structure of S. pyogenes Cas9 手法: X-RAY DIFFRACTION / 解像度: 2.62 Å PDB-4cmq: Crystal structure of Mn-bound S.pyogenes Cas9 手法: X-RAY DIFFRACTION / 解像度: 3.09 Å PDB-4ogc: Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii 手法: X-RAY DIFFRACTION / 解像度: 2.8 Å PDB-4oge: Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii 手法: X-RAY DIFFRACTION / 解像度: 2.201 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水 ChemComp-MN: Unknown entry ChemComp-ZN: Unknown entry ChemComp-SPD: SPERMIDINE / スペルミジン / スペルミジン ChemComp-ACT: ACETATE ION / 酢酸イオン / 酢酸塩
由来	streptococcus pyogenes (化膿レンサ球菌) Enterobacteria phage lambda (λファージ) actinomyces naeslundii (バクテリア)
キーワード	HYDROLASE (加水分解酵素) / DNASE (デオキシリボヌクレアーゼ) / RNA-GUIDED / IMMUNITY / CRRNA / GENOME EDITING (ゲノム編集) / ENDONUCLEASE (エンドヌクレアーゼ) / CRISPR-Cas (CRISPR) / Cas9 (Cas9) / HNH / RuvC / RNA-guided DNA endonuclease / cytoplasmic (細胞質)