EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Antibody recognition of the pandemic H1N1 Influenza virus hemagglutinin receptor binding site.
ジャーナル・号・ページ	J Virol, Vol. 87, Issue 22, Page 12471-12480, Year 2013
掲載日	2013年9月11日
著者	Minsun Hong / Peter S Lee / Ryan M B Hoffman / Xueyong Zhu / Jens C Krause / Nick S Laursen / Sung-Il Yoon / Langzhou Song / Lynda Tussey / James E Crowe / Andrew B Ward / Ian A Wilson
PubMed 要旨	Influenza virus is a global health concern due to its unpredictable pandemic potential. This potential threat was realized in 2009 when an H1N1 virus emerged that resembled the 1918 virus in ...Influenza virus is a global health concern due to its unpredictable pandemic potential. This potential threat was realized in 2009 when an H1N1 virus emerged that resembled the 1918 virus in antigenicity but fortunately was not nearly as deadly. 5J8 is a human antibody that potently neutralizes a broad spectrum of H1N1 viruses, including the 1918 and 2009 pandemic viruses. Here, we present the crystal structure of 5J8 Fab in complex with a bacterially expressed and refolded globular head domain from the hemagglutinin (HA) of the A/California/07/2009 (H1N1) pandemic virus. 5J8 recognizes a conserved epitope in and around the receptor binding site (RBS), and its HCDR3 closely mimics interactions of the sialic acid receptor. Electron microscopy (EM) reconstructions of 5J8 Fab in complex with an HA trimer from a 1986 H1 strain and with an engineered stabilized HA trimer from the 2009 H1 pandemic virus showed a similar mode of binding. As for other characterized RBS-targeted antibodies, 5J8 uses avidity to extend its breadth and affinity against divergent H1 strains. 5J8 selectively interacts with HA insertion residue 133a, which is conserved in pandemic H1 strains and has precluded binding of other RBS-targeted antibodies. Thus, the RBS of divergent HAs is targeted by 5J8 and adds to the growing arsenal of common recognition motifs for design of therapeutics and vaccines. Moreover, consistent with previous studies, the bacterially expressed H1 HA properly refolds, retaining its antigenic structure, and presents a low-cost and rapid alternative for engineering and manufacturing candidate flu vaccines.
リンク	J Virol / PubMed:24027321 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.55 - 23.0 Å
構造データ	EMDB-5731: Antibody Recognition of the Pandemic H1N1 Influenza Hemagglutinin Receptor Binding Site 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-5733: Antibody Recognition of the Pandemic H1N1 Influenza Hemagglutinin Receptor Binding Site 手法: EM (単粒子) / 解像度: 23.0 Å PDB-4m4y: Crystal structure of a 2009 H1N1 influenza virus hemagglutinin with a stabilization mutation HA2 E47G 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-4m5y: Crystal structure of broadly neutralizing Fab 5J8 手法: X-RAY DIFFRACTION / 解像度: 1.55 Å PDB-4m5z: Crystal structure of broadly neutralizing antibody 5J8 bound to 2009 pandemic influenza hemagglutinin, HA1 subunit 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-HOH: WATER / 水 ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル / ジエチレングリコール ChemComp-PG4: TETRAETHYLENE GLYCOL / テトラエチレングリコ-ル / 沈殿剤*YM / ポリエチレングリコール
由来	homo sapiens (ヒト) influenza a virus (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / viral envelope protein (カプシド) / viral fusion protein / stabilization mutant / IMMUNE SYSTEM (免疫系) / immunoglobulin fold / fragment antigen binding / influenza hemagglutinin receptor binding site / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / hemagglutinin (ヘマグルチニン) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性)