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-Structure paper
| タイトル | Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate. |
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| ジャーナル・号・ページ | Science, Vol. 391, Issue 6786, Page 694-699, Year 2026 |
| 掲載日 | 2026年2月12日 |
著者 | Donghwi Ko / Raili Ruonala / Alexandre Faille / Eva Hellmann / Hanna Help / Huili Liu / Ronni Nielsen / Anders Haakonsson / Nuria De Diego / Anja Paatero / Mariia V Shcherbii / Karolina Stefanowicz / Sanja Ćavar Zeljković / Tine Drud Lundager Rasmussen / Ondrej Novak / Zsuzsanna Bodi / Gugan Eswaran / Brecht Wybouw / Matthieu Bourdon / Cristina Urbez / Xiaonan Liu / Kari Salokas / Tiina Öhman / Tanya Waldie / Petri Törönen / Sedeer El-Showk / Martin Balcerowicz / Fabrice Besnard / Xiaomin Liu / Patrick Perkins / Serina Mazzoni-Putman / Julia P Vainonen / Maija Sierla / Mikko J Frilander / Susanne Mandrup / Teva Vernoux / Karin Ljung / Alejandro Ferrando / Miguel A Blazquez / Liisa Holm / Rupert Fray / Markku Varjosalo / Ottoline Leyser / Ville O Paavilainen / Ari Pekka Mähönen / Anna Stepanova / Jose Alonso / Steffen Heber / Robert Malinowski / Finn Kirpekar / Alan J Warren / Ykä Helariutta / ![]() |
| PubMed 要旨 | Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR- ...Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25 ribosomal RNA (rRNA). Residue mU2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation. |
リンク | Science / PubMed:41678627 |
| 手法 | EM (単粒子) |
| 解像度 | 1.82 - 2.25 Å |
| 構造データ | EMDB-51820, PDB-9h3g: EMDB-51899, PDB-9h6i: EMDB-52095, PDB-9hes: EMDB-52299, PDB-9hmw: |
| 化合物 | ![]() ChemComp-MG: ![]() ChemComp-K: ![]() ChemComp-TER: ![]() ChemComp-SPD: ![]() ChemComp-EPE: ![]() ChemComp-ZN: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | RIBOSOME / plant / thermospermine / methylation / rRNA modification / thaliana / polyamines / ovac / 3-methyluridine |
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