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タイトル | Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions. |
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ジャーナル・号・ページ | Structure, Vol. 18, Issue 8, Page 903-912, Year 2010 |
掲載日 | 2010年8月11日 |
著者 | Kazuyoshi Murata / Xiangan Liu / Radostin Danev / Joanita Jakana / Michael F Schmid / Jonathan King / Kuniaki Nagayama / Wah Chiu / |
PubMed 要旨 | Zernike phase contrast cryo-electron microscopy (ZPC-cryoEM) is an emerging technique that is capable of producing higher image contrast than conventional cryoEM. By combining this technique with ...Zernike phase contrast cryo-electron microscopy (ZPC-cryoEM) is an emerging technique that is capable of producing higher image contrast than conventional cryoEM. By combining this technique with advanced image processing methods, we achieved subnanometer resolution for two biological specimens: 2D bacteriorhodopsin crystal and epsilon15 bacteriophage. For an asymmetric reconstruction of epsilon15 bacteriophage, ZPC-cryoEM can reduce the required amount of data by a factor of approximately 3, compared with conventional cryoEM. The reconstruction was carried out to 13 A resolution without the need to correct the contrast transfer function. New structural features at the portal vertex of the epsilon15 bacteriophage are revealed in this reconstruction. Using ZPC cryo-electron tomography (ZPC-cryoET), a similar level of data reduction and higher resolution structures of epsilon15 bacteriophage can be obtained relative to conventional cryoET. These results show quantitatively the benefits of ZPC-cryoEM and ZPC-cryoET for structural determinations of macromolecular machines at nanometer and subnanometer resolutions. |
リンク | Structure / PubMed:20696391 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (単粒子) |
解像度 | 9.0 - 38.0 Å |
構造データ | EMDB-5203: EMDB-5204: EMDB-5206: EMDB-5207: EMDB-5208: EMDB-5209: |